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PhosphoPair STAT1 (Ser727) Antibody Set

Pricing & Availability
Clone
10C4B40; A15158B;
Regulatory Status
RUO
Other Names
Signal transducer and activator of transcription 1 (STAT1), Transcription factor ISGF-3 components p91/p84
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Product Citations
publications
PhosphoPair_STAT1-Ser727_Antibody_Set_1_05252021
Total cell lysates (15 µg total protein) from HeLa cells untreated (-) or treated (+) with nocodazole (200 ng/mL, 16 hours) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL (1:1000 dilution) purified anti-STAT1 phospho (Ser727) antibody (clone A15158B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Purified anti-STAT1 antibody (clone 10C4B40) (Cat. No. 661002) was used as a pan STAT1 loading control at 1.0 µg/mL (1:500 dilution). Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker.
  • PhosphoPair_STAT1-Ser727_Antibody_Set_1_05252021
    Total cell lysates (15 µg total protein) from HeLa cells untreated (-) or treated (+) with nocodazole (200 ng/mL, 16 hours) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL (1:1000 dilution) purified anti-STAT1 phospho (Ser727) antibody (clone A15158B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Purified anti-STAT1 antibody (clone 10C4B40) (Cat. No. 661002) was used as a pan STAT1 loading control at 1.0 µg/mL (1:500 dilution). Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker.
  • PhosphoPair_STAT1-Ser727_Antibody_Set_2_05252021
    Total cell lysates (15 µg total protein) from NIH/3T3 cells untreated (-) or treated (+) with UV radiation (20 mJ for 5 seconds) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) purified anti-STAT1 phospho (Ser727) antibody (clone A15158B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Purified anti-STAT1 antibody (clone 10C4B40) (Cat. No. 661002) was used as a pan STAT1 loading control at 1.0 µg/mL (1:500 dilution). Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker.
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Description

STAT1, also known as signal transduction and activator of transcription 1, is a ubiquitously expressed cytoplasmic protein and is activated in response to cytokine signaling, including IFN-α, IFN-γ, EGF, PDGF, and IL-6. Upon activation, STAT1 is phosphorylated by receptor-associated kinases, translocates to the nucleus, and functions as a transcription factor. Phosphorylation at serine 727 is required for full transcriptional activator function. Two isoforms of STAT1, with apparent molecular weights of 88 (STAT1α) and 91 (STAT1β) kD, exist as a result of alternative RNA processing. STAT1 is involved in IFN-mediated immune responses, and STAT1-deficient mice are highly sensitive to bacterial and viral infections.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
Product Application Clone Size Reactivity Isotype
Purified anti-STAT1 Antibody WB, IP 10C4B40 25 μg Human, Mouse Mouse IgG1, κ
Purified STAT1 Phospho (Ser727) Antibody WB, ICC, ICFC A15158B 25 μg Human, Mouse Mouse IgG1, κ


* For detailed information about each specificity, please refer to the datasheets of the individual products. 

Product Details

Verified Reactivity
Human, Mouse
Immunogen
Please refer to individual product datasheets for immunogen information
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality testeed
Recommended Usage

Each lot of antibodies in this set is quality control tested by Western blotting. For Western blotting, the suggested uses of these reagents are as follows:

Clone 10C4B40: 1.0 µg/mL
Clone A15158B: 0.5 - 2.5 µg/mL

It is recommended that the reagent be titrated for optimal performance for each application.

For recommended usage information for additional applications, please refer to the datasheet of the individual products.

Application Notes

The serine 727 phosphorylation site is only found in STAT1α; it is absent in STAT1β. Therefore, clone A15158B only detects a single band corresponding to the serine 727-phosphorylated α isoform, while clone 10C4B40 detects both α and β isoforms.

Antigen Details

Structure
Two isoforms of STAT1 exist as a result of alternative splicing. Isoform α is a 750 amino acid protein with a predicted molecular weight of 87 kD; the β isoform is a 712 amino acid protein with a predicted molecular weight of 83 kD.
Distribution

Ubiquitously expressed/Nucleus and cytosol

Function
Phosphorylated in response to cytokine signaling by receptor-associated kinases; translocates to the nucleus to act as a transcription factor. Mediates responses to type I (IFN-α/β) and II interferon (IFN-γ), EGF, PDGF, and IL-6.
Interaction
Forms a homodimer or heterodimers with other family members. Interacts with FAK, MCM3, MCM5, TRADD, BRCA1, KIT, IL-27R, IL-2Rβ, IL-2Rγ, IFNαβR, and c-Src.
Cell Type
Mesenchymal cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction, Stem Cells, Transcription Factors
Molecular Family
Nuclear Markers, Phospho-Proteins
Antigen References
  1. Durbin JE, et al. 1996. Cell. 84:443-50.
  2. Darnell JE Jr, et al. 1994. Science. 264:1415-21.
  3. Chen X, et al. 1998. Cell. 93:827-39.
  4. Ramana CV, et al. 2000. Oncogene. 19:2619-27.
Gene ID
6772 View all products for this Gene ID
UniProt
View information about STAT1 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 05/25/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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