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Prime-Step™ Prestained Broad Range Protein Ladder

Pricing & Availability
Regulatory Status
RUO
Other Names
protein ladder, protein marker, pre-stained protein ladder, pre-stained protein marker, protein molecular weight marker
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Product Citations
publications
PrimeStep_Protein_Ladder_1_Updated_042418
3µl Prime-Step™ Prestained Broad Range Protein Ladder was resolved by electrophoresis in different gel running systems, including 14%, 4-20% and 4-12% Tris-Glycine gel, 4-12% Bis-Tris gel (MOPS and MES running buffer), 3-8% Tris-Acetate gel and 10-20% Tricine gel. Then protein ladders were transferred to nitrocellulose membrane. Mw: molecular weight.
  • PrimeStep_Protein_Ladder_1_Updated_042418
    3µl Prime-Step™ Prestained Broad Range Protein Ladder was resolved by electrophoresis in different gel running systems, including 14%, 4-20% and 4-12% Tris-Glycine gel, 4-12% Bis-Tris gel (MOPS and MES running buffer), 3-8% Tris-Acetate gel and 10-20% Tricine gel. Then protein ladders were transferred to nitrocellulose membrane. Mw: molecular weight.
  • PrimeStep_Protein_Ladder_2_Updated_042418
    3µl Prime-Step™ Prestained Broad Range Protein Ladder (lane M) and total cell lysates (15µg total protein) was resolved by electrophoresis in 4-12% Bis-Tris gel (MOPS running buffer). Then protein ladders and total cell lysates were transferred to nitrocellulose (NC) and PVDF membrane followed by Ponceau red staining. Lane 1: Hela; Lane 2: Jurkat; Lane 3: NIH3T3 cells.
  • PrimeStep_Protein_Ladder_3_Updated_042418
    3µl Prime-Step™ Prestained Broad Range Protein Ladder (lane M) and 2µg recombinant proteins 1, 2, 3, with different molecular weights, was resolved by electrophoresis in 12% Bis-Tris gel (MES running buffer) followed by Coomassie blue staining.
  • PrimeStep_Protein_Ladder_4_Updated_042418
    3µl Prime-Step™ Prestained Broad Range Protein Ladder was resolved by electrophoresis in different gel running systems: 4-20% Tris-Glycine gel, 4-12% Bis-Tris gel (MES running buffer). Proteins were either stained with Coomassie blue, or transferred to nitrocellulose membrane followed by Ponceau red staining . Lane 1: Prime-Step™ Prestained Broad Range Protein Ladder; Lane 2: Competitor 1 protein ladder; Lane 3: Competitor 2 protein ladder.
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773301 50 µL £25
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773302 500 µL £85
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Description

The Prime-Step™ Prestained Broad Range Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 6.5 to 270 kDa. It has 7 blue-stained bands and 3 reference bands (2 orange bands at 30 kDa and 270 kDa and 1 green band at 52 kDa) when separated on Tris-glycine gel. The Prime-Step™ Prestained Broad Range Protein Ladder is designed for monitoring protein separation during SDS-PAGE electrophoresis, confirming Western blot transfer quality on membranes (PVDF or nitrocellulose membrane) and for approximating the size of target proteins on the gel or blot.

Key Product Features

  • Clear separation and sharp bands in different SDS-PAGE gel and buffer systems
  • Excellent transfer efficiency on PVDF and Nitrocellulose membrane for WB
  • Suitable for size detection during protein purification
  • Comparable MW detection with competitor’s protein ladders
  • Better performance for high molecular weight proteins
  • Supplied in the sample loading buffer and is ready to use
Product Details
Technical Data Sheet (pdf)

Product Details

Formulation
Sample loading buffer (62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3, 33% glycerol).
Preparation
Chromophore conjugated recombinant protein mixture supplied in sample loading buffer.
Storage & Handling
Upon receipt, store at -20°C.
Application

WB - Quality tested
Recommended Usage

• 3-5 μl per loading for clear visualization during electrophoresis on 15-well mini-gel or 20-well midi-gel, respectively.
• 3-5 μl per well for general Western transferring. 

The ladder is supplied in the sample loading buffer and is ready to use.

Application Notes

The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards.

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
Go To Top Version: 1    Revision Date: 03/21/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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