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Purified anti-4EBP1 Phospho (Thr70) Antibody

Pricing & Availability
Clone
A18010A (See other available formats)
Regulatory Status
RUO
Other Names
Eukaryotic Translation Initiation Factor 4E Binding Protein 1, Phosphorylated Heat- And Acid-Stable Protein Regulated By Insulin 1, PHAS-I
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A18010A_PURE_4EBP1-phospho-Thr70_Antibody_1_082619
Whole cell extracts (15 µg protein) from untreated (-) or LY294002-treated (+, 100 µM for 1 hour) HEK-293 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-4EBP1 Phospho (Thr70) Antibody, clone A18010A, for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • A18010A_PURE_4EBP1-phospho-Thr70_Antibody_1_082619
    Whole cell extracts (15 µg protein) from untreated (-) or LY294002-treated (+, 100 µM for 1 hour) HEK-293 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-4EBP1 Phospho (Thr70) Antibody, clone A18010A, for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
Cat # Size Price Quantity Check Availability Save
619601 25 µg £77
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619602 100 µg £182
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Description

In eukaryotes, the mTOR kinase signaling pathway links cell growth and proliferation with nutrient availability. Under nutrient-deplete conditions, eukaryotic translation initiations factor 4E-binding protein 1 (4EBP1) binds the general translation factor eIF4E, resulting in an attenuation of cap-dependent translation. Under nutrient-replete conditions, mTORC1 and mTORC2 complexes phosphorylate 4EBP1 at Threonine 70 and disrupt the formation of the 4EBP1-eIF4E complex, thereby alleviating the repressive function of 4EBP1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide corresponding to human 4EBP1 phosphorylated at Threonine 70
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone has not been tested for ICC and IP.

RRID
AB_2814468 (BioLegend Cat. No. 619601)
AB_2814468 (BioLegend Cat. No. 619602)

Antigen Details

Structure
4EBP1 is a 118 amino acid protein with a predicted molecular weight of 13 kD.
Distribution

Cytosol and nucleus/ubiquitously expressed

Function
Regulation of global translation
Biology Area
Cell Biology, Cell Proliferation and Viability, Protein Synthesis, Signal Transduction
Molecular Family
Phospho-Proteins
Antigen References
  1. Brunn GJ, et al. 1997. Nature. 277:99.
  2. Hara K, et al. 2002. Cell. 110:177.
  3. Ekim B, et al. 2011. Mol. Cell Biol. 31:2787.
Gene ID
1978 View all products for this Gene ID
UniProt
View information about 4EBP1 on UniProt.org
Go To Top Version: 1    Revision Date: 08/26/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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