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Purified anti-Adenomatous polyposis coli (APC) Antibody

Pricing & Availability
Clone
Ali 12-28 (See other available formats)
Regulatory Status
RUO
Other Names
APC, BTPS2, DP2, DP2.5, DP3, PPP1R46, WNT signaling pathway regulator, adenomatosis polyposis coli tumor suppressor, deleted in polyposis 2.5, protein phosphatase 1, regulatory subunit 46, truncated adenomatosis polyposis coli
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
Ali-12-28_PURE_APC_Antibody_092917
Western blot of anti-Adenomatous polyposis coli protein (APC) antibody (clone Ali 12-28) and isotype-matched IgG1 control. Blots were incubated with 5µg/ml of anti-APC (left) or mouse IgG1 isotype control (right) overnight at 4°C, followed by the incubation with an HRP goat anti-mouse IgG secondary antibody (clone Poly4053). Enhanced chemiluminescence was used as the detection system. MW: molecular weight marker; human brain lysates: 20µg.
  • Ali-12-28_PURE_APC_Antibody_092917
    Western blot of anti-Adenomatous polyposis coli protein (APC) antibody (clone Ali 12-28) and isotype-matched IgG1 control. Blots were incubated with 5µg/ml of anti-APC (left) or mouse IgG1 isotype control (right) overnight at 4°C, followed by the incubation with an HRP goat anti-mouse IgG secondary antibody (clone Poly4053). Enhanced chemiluminescence was used as the detection system. MW: molecular weight marker; human brain lysates: 20µg.
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850801 25 µg £97
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850802 100 µg £241
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Description

The APC gene provides instructions for making the APC protein, which plays a critical role in several cellular processes. The APC protein acts as a tumor suppressor, which means that it keeps cells from growing and dividing too fast or in an uncontrolled way. It helps control how often a cell divides, how it attaches to other cells within a tissue, and whether a cell moves within or away from a tissue. This protein also helps ensure that the number of chromosomes in a cell is correct following cell division. The APC protein accomplishes these tasks mainly through association with other proteins, especially those that are involved in cell attachment and signaling.

One protein with which APC associates is beta-catenin. Beta-catenin helps control the activity (expression) of particular genes and promotes the growth and division (proliferation) of cells and the process by which cells mature to carry out specific functions (differentiation). Beta-catenin also helps cells attach to one another and is important for tissue formation. Association of APC with beta-catenin signals for beta-catenin to be broken down when it is no longer needed.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
N-terminal APC-MBP fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy1.

Application References

(PubMed link indicates BioLegend citation)
  1. Boehlke C, et al. 2013. PLoS One. 8(5):e62165. (IF)
  2. Hadjihannas MV, et al. 2012. EMBO Rep. 13(4):347-54. (WB)
  3. Tanneberger K, et al. 2011. J. Biol. Chem. 286(22):19204-14. (WB)
RRID
AB_2716017 (BioLegend Cat. No. 850801)
AB_2716017 (BioLegend Cat. No. 850802)

Antigen Details

Structure
APC is a 2843 amino acid protein with a molecular weight of 311 kD.
Distribution

Tissue distribution: expressed in wide variety of normal tissues.
Cellular distribution: cellular junctions, cytoplasm and membranous expression.

Function
APC is a tumor suppressor involved in Wnt signaling. It is also involved in additional processes including cell migration and adhesion, transcriptional activation and apoptosis.
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Tumor Suppressors
Antigen References

1. Barth AI, et al. 2008. Semin. Cell. Dev. Biol. 19(3):245-51.

Gene ID
324 View all products for this Gene ID
UniProt
View information about Adenomatous polyposis coli on UniProt.org
Go To Top Version: 2    Revision Date: 01/15/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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