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Purified anti-Aggrecan Antibody

Pricing & Availability
Clone
M4004A03 (See other available formats)
Regulatory Status
RUO
Other Names
Aggrecan 1, AGC1, Aggrecan Proteoglycan, Chondroitin Sulfate Proteoglycan 1, Cartilage-Specific Proteoglycan Core Protein, CSPG1, MSK16, CSPGCP, SEDK, and CSPCP
Isotype
Mouse IgG1, κ
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Product Citations
publications
M4004A03_Purified_Aggrecan_Antibody_WB_022015
Western blot analysis of 50ng of recombinant human Aggrecan (G1-IGD-G2) under reducing (lane 1) or non-reducing (lane 2) using anti-Aggrecan antibody (clone M4004A03).
  • M4004A03_Purified_Aggrecan_Antibody_WB_022015
    Western blot analysis of 50ng of recombinant human Aggrecan (G1-IGD-G2) under reducing (lane 1) or non-reducing (lane 2) using anti-Aggrecan antibody (clone M4004A03).
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674902 100 µg £182
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Description

Aggrecan, also known as aggrecan 1, is the major proteoglycan in the articular cartilage. This high molecular weight protein exhibits a bottlebrush structure, in which chondroitin sulfate and keratan sulfate chains are attached to an extended protein core. Aggrecan plays an important role in the proper functioning of articular cartilage by providing a hydrated gel structure through its interaction with hyaluronan and link proteins. Aggrecan is expressed by chondrocytes and is found to be crucial in chondroskeletal morphogenesis during development. Mutations in this gene may be involved in skeletal dysplasia and spinal cord degeneration. Structurally, aggrecan is composed of two globular domains (G1 and G2) at the N-terminal end and one globular domain (G3) at the C-terminal end, which are separated by a large extended domain (CS) heavily modified with glycosaminoglycans. G1 and G2 domains of aggrecan are separated by the interglobular domain (IGD). Biologically, the G1 domain interacts with hyaluronan acid and link proteins, which forms stable ternary complexes in the extracellular matrix (ECM). The G2 domain is homologous to the tandem repeats of G1 and link proteins, and is involved in product processing. G3 makes up the carboxyl terminus of the core protein, and enhances glycosaminoglycan modification and product secretion. Also, the G3 domain links the proteoglycan aggregates to the ECM proteins, which are fibulins and tenascins. The degradation of aggrecan appears to initiate at the C-terminus. The population of aggrecan molecules without the G3 domain increases with aging.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Aggrecan produced in 293E cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody works for Western blotting under non-reducing conditions.

RRID
AB_2565486 (BioLegend Cat. No. 674902)

Antigen Details

Structure
2415 amino acids with a predicted molecular weight of approximately 250 kD.
Distribution

Chondrocytes and embryonic brain glia.

Function
Plays a key role in neural development, glial scar formation, cell adhesion, cell growth, receptor binding, cell migration, and interaction with other extracellular matrix constituents.
Ligand/Receptor
Aggrecan is an enzymatic substrate of MMPs and ADAMT11. It interacts with versican, which is a large ECM proteoglycan.
Biology Area
Cell Biology, Signal Transduction
Antigen References

1. Doege KJ, et al. 1991. J. Biol. Chem. 266:894.
2. Nap RJ and Szleifer I. 2008. Biophys. J. 95:4570.
3. Roughley P, et al. 2006. Eur. Cell Mater. 11:1.
4. Kiani C, et al. 2002. Cell Res. 12:19-32.
5. Melin Fürst C, et al. 2013. PLoS One 8:e61407.

Gene ID
176 View all products for this Gene ID
UniProt
View information about Aggrecan on UniProt.org
Go To Top Version: 2    Revision Date: 06/13/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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