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Purified anti-AGR2 Antibody

Pricing & Availability
Clone
W19087A (See other available formats)
Regulatory Status
RUO
Other Names
Anterior Gradient 2, Protein Disulphide Isomerase Family Member, HAG-2, PDIA17, XAG-2, HPC8, Epididymis Secretory Protein Li 116, Secreted Cement Gland Homolog, GOB-4, HEL-S-116
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W19087A_PURE_AGR2_Antibody_1_111320
Whole cell extracts (15 µg total protein) from HeLa (negative control), A549 (low expressor positive control), and MCF7 (high expressor positive control) lysates were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 2.0 µg/mL (1:250 dilution) purified anti-AGR2 antibody (clone W19087A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker. Predicted AGR2 expression was obtained from Human Protein Atlas.
  • W19087A_PURE_AGR2_Antibody_1_111320
    Whole cell extracts (15 µg total protein) from HeLa (negative control), A549 (low expressor positive control), and MCF7 (high expressor positive control) lysates were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 2.0 µg/mL (1:250 dilution) purified anti-AGR2 antibody (clone W19087A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker. Predicted AGR2 expression was obtained from Human Protein Atlas.
  • W19087A_PURE_AGR2_Antibody_2_111320
    HeLa cells (negative control, panel A) and A549 cells (positive control, panel B) were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with 2.0 µg/mL purified anti-AGR2 antibody (clone W19087A) overnight at 4°C followed by incubation with Alexa Fluor® 594 goat anti-rat IgG antibody (Cat. No. 405422) at 2.0 µg/mL. Nuclei were counterstained with DAPI and the image was captured with a 60X objective.
  • W19087A_PURE_AGR2_Antibody_3_111320
    Whole cell extracts (250 µg total protein) prepared from MCF7 cells were immunoprecipitated overnight with 2.5 µg of purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400501) or purified anti-AGR2 antibody (clone W19087A). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a rabbit control antibody against a separate epitope of AGR2. Lane M: Molecular weight marker.
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943101 25 µg £81
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943102 100 µg £201
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Description

AGR2 is a disulfide isomerase that catalyzes protein folding and thiol-disulfide exchange reactions in the ER lumen. It can also serve as a chaperone for secretory proteins, and is essential for maturation of cysteine-rich transmembrane receptors, including the intestinal glycoprotein mucin. In addition to its role in ER homeostasis, AGR2 can also exert pro-oncogenic function. In many tumors, AGR2 is overexpressed and secreted into the tumor microenvironment, where it disrupts epithelial cell to cell adhesion, promotes invasive structures, and mediates loss of cell polarity. AGR2 is highly upregulated in tamoxifen-treated cells and plays a crucial role in mediating resistance to chemotherapeutic agents.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human AGR2 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 2.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was tested for ICC on A549 cells fixed with 4% PFA and permeabilized with either Triton X-100 or methanol. While both methods were compatible with AGR2 staining, a significantly stronger signal was attained with Triton X-100 permeabilization.

AGR2 staining was highly variable in A549 cells, with many cells lacking any detectable signal. This result was consistent with internal data generated with an anti-AGR2 control antibody.

RRID
AB_2888903 (BioLegend Cat. No. 943101)
AB_2888903 (BioLegend Cat. No. 943102)

Antigen Details

Structure
AGR2 is a 175 amino acid protein with a predicted molecular weight of 20 kD.
Distribution

Primarily expressed in epithelial tissues/Endoplasmic reticulum

Function
ER chaperone, protein folding, disulfide isomerase
Biology Area
Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Structure, Protein Misfolding and Aggregation, Protein Synthesis
Antigen References

1. Chevet E, et al. 2013. Oncogene. 32:2499-509.
2. Fessart D, et al. 2016. Elife. 5:13887.
3. Hengel SM, et al. 2011. J Proteome Res. 10:4567-78.

Gene ID
10551 View all products for this Gene ID
UniProt
View information about AGR2 on UniProt.org
Go To Top Version: 1    Revision Date: 11/13/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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