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Purified anti-AIM2 Antibody

Pricing & Availability
Clone
3B10 (See other available formats)
Regulatory Status
RUO
Other Names
Absent in melanoma 2 (AIM2), PYHIN4, interferon-inducible protein AIM2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
3B10_Purified_AIM2_Antibody_WB_050418
Total lysates (15 µg protein) from 293E (Lane 1) and Daudi (Lane 2) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 0.5 µg/mL Purified anti-AIM2 Antibody, clone 3B10 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-AIM2 Antibody or 1:5000 diluted Direct-Blot™ HRP anti-β-Actin Antibody, clone 2F1-1(lower). Lane M: Molecular weight ladder
  • 3B10_Purified_AIM2_Antibody_WB_050418
    Total lysates (15 µg protein) from 293E (Lane 1) and Daudi (Lane 2) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 0.5 µg/mL Purified anti-AIM2 Antibody, clone 3B10 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-AIM2 Antibody or 1:5000 diluted Direct-Blot™ HRP anti-β-Actin Antibody, clone 2F1-1(lower). Lane M: Molecular weight ladder
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652801 25 µg £105
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652802 100 µg £238
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Description

AIM2 is one of the human IFN-inducible proteins, encoding a 39 kD protein. It contains a 200 amino acid repeat, which is a signature of the HIN-200 family (hematopoietic IFN-inducible nuclear proteins). Expression of AIM2 can inhibit cell growth and tumor formation, possibly through inducing apoptosis and suppressing nuclear factor-κ B transcriptional activity and desensitizing tumor necrosis factor-alpha-mediated nuclear factor-κ B activation. Furthermore, the gene for AIM2 shows a high frequency of mutations that are associated with microsatellite unstable colorectal cancers. AIM2 plays a critical role in the activation of caspase-1, the protease responsible for the processing of pro-inflammatory cytokines IL-1β and IL-18. AIM2 is also essential for mediating inflammatory reactions triggered by cytoplasmic DNA. AIM2 is an important sensor for different pathogens through the binding of cytoplasmic double-stranded DNA through HIN-200 domain and formation of inflammatsomes including ASC/AIM2. This complex then triggers caspase-1 activation. Recent studies also show that AIM2/ASC complex acts as a novel caspase-8 activation platform.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant fragment, corresponding to amino acids 1-186 of human AIM2 with a GST tag.
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, 0.09% NaN3.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP - Reported by the developer, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5-2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1, immunofluorescence staining3 and immunoprecipitation2.

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Pierini R, et al. 2012. Cell Death Differ. 19:1709. (WB)
  2. Fernandez-Alnemri T, et al. 2009. Nature 7237:509. (IP)
  3. Sagulenko V, et al. 2013. Cell Death Differ. 9:1149. (IF)
Product Citations
  1. Su S et al. 2018. Cell. 175(2):442-457 . PubMed
RRID
AB_2561540 (BioLegend Cat. No. 652801)
AB_2561540 (BioLegend Cat. No. 652802)

Antigen Details

Structure
343 amino acids with predicted molecular weight of 39 kD.
Distribution

Cytosol.

Function
Tumor suppressor which may act by repressing NF-κ B transcriptional activity.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity
Antigen References

1. DeYoung KL, et al. 1997. Oncogene 15:453.
2. Choubey D, et al. 2000. FEBS Lett. 474:38.
3. Chen IF, et al. 2006. Mol. Cancer Ther. 5:1.
4. Roberts TL, et al. 2009. Science 323:1057.
5. Jones JW, et al. 2010. Proc. Natl. Acad. Sci. USA 107:9771.
6. Fernandes-Alnemri T, et al. 2010. Nat. Immunol. 11:385.
7. Schroder K and Tschopp J. 2010. Cell 140:821.
8. Jin T, et al. 2012. Immunity 36:561.

Gene ID
9447 View all products for this Gene ID
UniProt
View information about AIM2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 05/07/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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