- Clone
- Poly23A04 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Protein kinase B alpha, PRK-BA, PKB, Serine/Threonine specific kinase RAC alpha, Protein kinase Akt, RAC
- Isotype
- Rabbit Polyclonal IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![Poly23A04_PURE_AKT_WB_010424 Poly23A04_PURE_AKT_WB_010424](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/Poly23A04_PURE_AKT_WB_010424.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
Whole cell extracts (15 µg total protein) from serum-starved HeLa cells untreated (-) or treated (+) with IGF-1 (100 ng/mL, 30 min), and from serum-starved NIH/3T3 cells untreated (-) or treated (+) with PDGF-BB (200 ng/mL, 15 min) were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL (1:1000 dilution) of Purified anti-AKT Phospho (Ser473) (clone Poly23A04) for 2 hours at room temperature (1st and 3rd blots). Proteins were visualized by chemiluminescence detection using HRP Goat anti-rabbit IgG (Cat. No. 406401) at a 1:3000 dilution. Equal AKT Phospho (Ser473) loading was confirmed by probing membranes with pan Purified anti-AKT1 (clone O94E10) (Cat. No. 680302) at a 1:500 dilution (2nd and 4th blots). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
627451 | 50 µL | £144 | ||||
627452 | 200 µL | £360 |
AKT (also known as protein kinase B alpha) is a 60 kD serine/threonine-specific kinase containing a pleckstrin domain. AKT plays a critical role in controlling survival and apoptosis. This kinase is ubiquitously expressed and translocates to the membrane upon cell activation. It is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3-kinase. AKT is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2, responsible for phosphorylation of AKT at Ser473, has been identified as a mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with RICTOR and SIN1. Activated AKT phosphorylates a wide range of substrates including transcription factors (e.g. FOXO1), kinases (GSK-3beta, Raf-1, ASK, Chk1) and other proteins with important signaling roles (e.g. Bad, MDM2). AKT promotes cell survival by inhibiting apoptosis through phosphorylation of several targets, including Bad, forkhead transcription factor, c-Raf and caspase-9. AKT also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor. More importantly, AKT phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- Synthetic peptide of human AKT phosphorylated at Ser473
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested dilution of this reagent is 1:1000 - 1:4000. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This clone was tested by Western Blot using lysates prepared from HeLa cells untreated or treated with IGF-1, and NIH/3T3 cells untreated or treated with PDGF-BB.
- RRID
-
AB_3097484 (BioLegend Cat. No. 627451)
AB_3097484 (BioLegend Cat. No. 627452)
Antigen Details
- Structure
- AKT is a 541 amino acid protein with a predicted molecular weight of 62 kD
- Distribution
-
Ubiquitously expressed, translocates to the membrane upon activation
- Function
- Catalytically inactive multimeric complex. AKT is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473 by PDK2.
- Interaction
- Interacts through pleckstrin homology domain with second messengers. Interacts with AKTIP and CDKN1B.
- Biology Area
- Cell Adhesion, Cell Biology, Cell Death
- Molecular Family
- Phospho-Proteins, Protein Kinases/Phosphatase
- Antigen References
-
- Jacinto E, et al. 2006. Cell. 127:125-37.
- Inoki K, et al. 2002. Nat Cell Biol. 4:648-57.
- Franke TF, et al. 1995. Cell. 81:727-36.
- Brunet A, et al. 1999. Cell. 96:857-68.
- Yang WL, et al. 2010. Cell Cycle. 9:487-97.
- Gene ID
- 207 View all products for this Gene ID
- UniProt
- View information about AKT on UniProt.org
Other Formats
View All AKT Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-AKT Phospho (Ser473) | Poly23A04 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-AKT Phospho (Ser473)
Whole cell extracts (15 µg total protein) from serum-starved...
Follow Us