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Purified anti-Alpha β Crystallin Antibody (Previously Covance catalog# PRB-105P)

Pricing & Availability
Clone
Poly9079 (See other available formats)
Regulatory Status
RUO
Other Names
Alpha-crystallin B chain, alpha B crystallin, heat shock protein beta-5, rosenthal fiber component, heat-shock 20 kD like-protein, renal carcinoma antigen NY-REN-27
Previously
Covance Catalog# PRB-105P
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
1_Poly9079_Purified_abcrystallin_Antibody_IHC_032415
Staining of Alpha B Crystallin, on formalin fixed paraffin embedded rat heart.
  • 1_Poly9079_Purified_abcrystallin_Antibody_IHC_032415
    Staining of Alpha B Crystallin, on formalin fixed paraffin embedded rat heart.
  • 2_Poly9079_PURE_Alpha_Crystalin_B_Antibody_062321.png
    Western blot of purified anti-alpha β crystalline antibody (clone Poly9079). Total tissue lysates (15 µg total protein) from human brain (lane 1), mouse brain (lane 2), and rat brain (lane 3) were resolved by 4-12% Bis-Tris gel electrophoresis, and then transferred to a PVDF membrane. The membrane was probed with 1.0 µg/mL of primary antibody overnight at 4°C, followed by incubation with HRP donkey anti-rabbit IgG antibody (Cat. No. 406401) at a 1:3000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
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907903 25 µL £61
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Description

Alpha B Crystallin (CryAB) is a member of the small heat shock protein (HSP20) family.  Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. CryAB can be induced by heat shock, ischemia and oxidation. The protein is known to protect cells from stress damage. It may also play an important role in the Golgi reorganization during cell division. CryAB is also known to be associated with a number of neurodegenerative diseases such as Alzheimer’s disease and has been implicated in breast tumors of patients with shorter survival, suggesting that it may be a useful marker in identifying aggressive breast cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC, WB
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For formalin-fixed paraffin-embedded immunohistochemical staining, a concentration range of 1 - 3 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.1 - 1 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

 

Application Notes

This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC).

*Predicted MW = 22 kD


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Thoua NM, et al. 2000. J Neuroimmunol. 104:47.
  2. Dasgupta S, et al. 1992. Exp Eye Res. 54:461.
  3. Bhat SP, et al. 1991. Euro J Biochem. 102:775.
RRID
AB_2734684 (BioLegend Cat. No. 907903)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Gene ID
1410 View all products for this Gene ID
UniProt
View information about Alpha beta Crystallin on UniProt.org
Go To Top Version: 5    Revision Date: 06/23/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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