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Purified anti-α Smooth Muscle Actin Antibody

Pricing & Availability
Clone
1A4 (See other available formats)
Regulatory Status
RUO
Other Names
Actin, aortic smooth muscle (αSMA), alpha 2 actin (ACTA2), alpha-cardiac actin, growth-inhibiting gene 46, cell growth-inhibiting gene 46 protein
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
a. 1A4_PURE_Alpha-Smooth_-IHC-P-_032323
IHC staining of purified anti-α smooth muscle actin (clone 1A4) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using 1X Tris-Buffered Saline (final concentration 0.05M) with Tween-20 (Cat. No. 925501), the tissue was incubated without (panels A and C) and with (panels B and D) 5 µg/mL of primary antibody followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG antibody (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (panels A and B) (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 µm
  • a. 1A4_PURE_Alpha-Smooth_-IHC-P-_032323
    IHC staining of purified anti-α smooth muscle actin (clone 1A4) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using 1X Tris-Buffered Saline (final concentration 0.05M) with Tween-20 (Cat. No. 925501), the tissue was incubated without (panels A and C) and with (panels B and D) 5 µg/mL of primary antibody followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG antibody (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (panels A and B) (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 µm
  • b. 1A4_PURE_Alpha-Smooth_-ICC-_032323
    HeLa cells were fixed with ice-cold methanol and blocked with 5% FBS for 1 hour at room temperature. The cells were then stained with 1.25 µg/mL of purified anti-α smooth muscle actin (clone 1A4), followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 60X objective. Scale bar: 50 µm
  • c. 1A4_PURE_Alpha-Smooth_-WB-_032323
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL of purified anti-α smooth muscle actin (clone 1A4) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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614851 25 µg £86
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614852 100 µg £213
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Description

Cytoplasmic actins, which belong to the microfilament system of cytoskeleton proteins, are some of the most conserved eukaryotic proteins expressed in mammals. α-smooth muscle actin is widely expressed in contractile cells, most notably the mural cells that line arteries and arterioles. Overexpression of α-smooth muscle actin is a hallmark of fibrosis, a thickening or scarring of damaged tissue.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
N-terminal synthetic peptide of alpha-smooth muscle actin coupled to keyhole limpet haemocyanin (KLH).
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
ICC, WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration of 1.25 μg/mL is recommended. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Previously Covance catalog# MMS-466S.

When used in ICC testing, we recommend fixing and permeabilizing with ice-cold methanol.

RRID
AB_2941620 (BioLegend Cat. No. 614851)
AB_2941620 (BioLegend Cat. No. 614852)

Antigen Details

Structure
α smooth muscle actin is a 377 amino acid protein with a predicted molecular weight of 42 kD.
Distribution

Cytoplasmic, cytoskeleton

Function
Cell motility
Interaction
α smooth muscle actins bind to myosins to produce motive force
Cell Type
Mesenchymal Stem Cells, Monocytes
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Microfilaments
Antigen References
  1. Brennan PA, et al. 2000. J Oral Pathol Med. 29:279-83.
  2. Rizeq MN, et al. 1994. Hum Pathol. 25:671-7.
  3. Schmitt-Graff A, et al. 1991. Am J Pathol. 138::1233-42
  4. Roholl PJM, et al. 1990. Hum Pathol. 21:1269-74.
  5. Alberts B, et al. 1989. Eds Molecular biology of the cell. 2:613.
  6. Skalli O, et al. 1986. J Cell Biol. 103:2787-96.
Gene ID
59 View all products for this Gene ID
UniProt
View information about alpha Smooth Muscle Actin on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 03/23/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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