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Purified anti-Amphiregulin Antibody

Pricing & Availability
Clone
1501107 (See other available formats)
Regulatory Status
RUO
Other Names
Amphiregulin (AR), AREG, Schwannoma-derived growth factor (SDGF), Colorectum cell-derived growth factor (CRDGF)
Isotype
Rat IgG2a, κ
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Product Citations
publications
1501107_PURE_Amphiregulin_Antibody_WB_031416
Western blot analysis of 50ng of Recombinant Mouse Amphiregulin (carrier-free) under non-reducing condition. Purified rat anti-mouse Amphiregulin antibody (clone 1501107) was used, followed by HRP Goat anti-rat IgG (minimal x-reactivity) antibody.
  • 1501107_PURE_Amphiregulin_Antibody_WB_031416
    Western blot analysis of 50ng of Recombinant Mouse Amphiregulin (carrier-free) under non-reducing condition. Purified rat anti-mouse Amphiregulin antibody (clone 1501107) was used, followed by HRP Goat anti-rat IgG (minimal x-reactivity) antibody.
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684802 100 µg £197
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Description

Amphiregulin was initially identified in the conditioned medium of human mammary gland MCF-7 cells treated with TPA. The mouse gene was cloned from the androgen-dependent SC2G cell line derived from Shionogi mouse mammary carcinoma SC115. It belongs to the EGF family of proteins that includes EGF, TGF-α, heparin-binding EGF like-growth factor (HB-EGF), epigen, epiregulin, and betacellulin. Mouse amphiregulin is derived from a 248 amino acid transmembrane precursor and it has 66% identity to the human protein. All the EGF family members are synthesized as type I membrane protein precursors, which can undergo proteolytic cleavage at the plasma membrane to release a mature soluble ectodomain. ADAM17 (TACE) has an important role in ectodomain shedding of amphiregulin, TNF-α, and HB-EGF. This cleavage is a key step in the control of ligand availability and receptor activation. It is stimulated by physiological and pharmacological agonists, including TPA, calcium ionophores, GPCR ligands, cytokines, and growth factors. Amphiregulin is a paracrine regulator of estrogen action during ductal morphogenesis (mammary gland development), and it has been associated with breast cancer initiation and progression. Amphiregulin is elevated in psoriatic lesional skin, gastrointestinal carcinomas, colorectal cancer, and hepatocelular carcinoma tissues. In addition, FOXP3+ Tregs express EGFR under inflammatory conditions, and amphiregulin enhances regulatory T cell-suppressive function in vitro and in vivo in a colitis and tumor vaccination model.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Purified mouse Amphiregulin, amino acids (Ser94-Lys191) (Accession# NM_009704) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody works for Western blotting under non-reducing conditions.

RRID
AB_2572176 (BioLegend Cat. No. 684802)

Antigen Details

Structure
248 amino acids with a predicted molecular weight of approximately 27 kD.
Distribution

Fibroblasts, epithelial cells, carcinoma cell lines, T cell subsets, naive and memory CD4 and CD8 T cells, Th1 and Th2 cells, mast cells, eosinophils, and basophils.

Function
Amphiregulin induces proliferation of fibroblasts, keratinocytes, and normal and malignant epithelial cells. It acts in normal development, organogenesis, and tumorigenesis. Ampiregulin release is mediated by IL-1β in fibroblast-like synoviocytes in rheumatoid arthritis patients. It is induced by IL-3 and IL-5 in basophils and eosinophils, respectively.
Interaction
Keratinocytes, epithelial cells, fibroblasts, carcinoma cells, and FOXP3 Tregs.
Ligand/Receptor
EGFR (HER1).
Cell Type
Basophils, Eosinophils, Epithelial cells, Fibroblasts, Mast cells, T cells, Th1, Th2, Tregs
Biology Area
Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability, Immunology, Signal Transduction
Antigen References

1. Plowman GD, et al. 1990. Mol. Cell Biol. 10:1969.
2. Sonoda H, et al. 1992. Biochem. Biophys. Res. Commun. 185:103.
3. Le Gall SM, et al. 2003. J. Biol. Chem. 278:45255.
4. Berasain C, et al. 2007. Cancer Letters 254:30.
5. Busser B, et al. 2011. Biochem. Biophys. Acta. 2:119.
6. Latasa MU, et al. 2012. PLoS One 7:e52711.
7. Qi Y, et al. 2012. PLoS One 7:e39072.
8. Zaiss DM, et al. 2013. Immunity 38:275.
9. Taira N, et al. 2014. Proc. Natl. Acad. Sci. USA 111:717.

Gene ID
11839 View all products for this Gene ID
UniProt
View information about Amphiregulin on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 03/28/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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