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Purified anti-Angiopoietin-2 Antibody

Pricing & Availability
Clone
M5203F01 (See other available formats)
Regulatory Status
RUO
Other Names
ANG-2, ANGPT2, AGPT2
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
M5203F01_Purified_Angiopoietin2_Antibody_WB_122215
Recombinant human angiopoietin-2 (50 ng, lane 1) and HUVEC cell lysate (30 µg, lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-Angiopoietin-2 (clone M5203F01) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Exposure time was ten seconds for lane 1 and five minutes for lane 2.
  • M5203F01_Purified_Angiopoietin2_Antibody_WB_122215
    Recombinant human angiopoietin-2 (50 ng, lane 1) and HUVEC cell lysate (30 µg, lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-Angiopoietin-2 (clone M5203F01) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Exposure time was ten seconds for lane 1 and five minutes for lane 2.
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682702 100 µg £182
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Description

Angiopoietin-2, also known as ANGPT2, belongs to a family of vascular growth factors. It plays essential roles in embryonic and postnatal angiogenesis. The structure of the mature form of Angiopoietin-2 contains an N-terminal coiled-coil domain, which mediates multimerization of the protein, along with a C-terminal fibrinogen-like domain, which mediates the receptor binding. Both Angiopoietin-1 and Angiopoietin-2 are ligands for the receptor tyrosine kinase, Tie-2. Angiopoietin-2 appears to exist predominantly as a homodimer, but it is also capable of forming multimers while Angiopoietin-1 predominantly exists as multimers. It has been shown that Angiopoietin-2 is able to promote vascular leakage and neutrophil migration. It can also sensitize TNF-α-induced monocyte adhesion. Thus, it is considered a proinflammatory factor. Although Angiopoietin-2 is not required for arterial vascular development, its expression in the tissue suggests that it may play a role in the maintenance of these vessels. This conclusion is also supported by the observation that high concentrations of Angiopoietin-2 activate the same survival signaling pathway as Angiopoietin-1 to prevent endothelial cell apoptosis via Tie-2/Akt. Additionally, Angiopoietin-2 functions as an agonist in the absence of Angiopoietin-1, but it acts as an antagonist in its presence. Human Angiopoietin-2 shares an 86% amino acid sequence homology with its murine counterpart.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Angiopoietin-2, amino acids (Tyr19-Phe496) (Accession# NP_001138), was expressed with a C-terminal His tag in CHO cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2566672 (BioLegend Cat. No. 682702)

Antigen Details

Structure
Oligomer.
Distribution

Secreted protein, low abundance in nucleus. Placenta, uterus, ovaries, and vascular endothelial cells.

Function
Angiopoietin-2 plays a role in angiogenesis and monocyte adhesion. Hyperoxia induces ANGPT2 expression in the lung epithelial cells.
Interaction
Monocytes and endothelial cells.
Ligand/Receptor
Tie-2.
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Augustin HG, et al. 2009. Nat. Rev. Mol. Biol. 10:165.
2. Ward NL, Dumont DJ. 2002. Semin. Cell Dev. Biol. 1:19-27.
3. Roviezzo F, et al. 2005. J. Pharmacol. Exp. Ther. 314:738.
4. Sturn DH, et al. 2005. Microcirculation 12:393.
5. Murdoch C, et al. 2007. J. Immunol. 178:7405.
6. Yuan HT, et al. 2009. Mol. Cell Biol. 29:2011.
7. Procopio WN, et al. 1999. J. Biol. Chem. 274:30196.
8. Bhandari V, et al. 2006. Nature Med. 12:1286.

Gene ID
285 View all products for this Gene ID
UniProt
View information about Angiopoietin-2 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 12/22/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

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