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Purified anti-Ankyrin-R Antibody

Pricing & Availability
Clone
N388A/10 (See other available formats)
Regulatory Status
RUO
Other Names
ANK1, Ankyrin-R
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
N388A-10_Purified_Ankyrin-R_Antibody_1_112018
Western blot of purified anti-Ankyrin-R antibody (clone N388A/10). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain lysate; Lane 3: 20 µg of rat brain lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N388A-10_Purified_Ankyrin-R_Antibody_1_112018
    Western blot of purified anti-Ankyrin-R antibody (clone N388A/10). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain lysate; Lane 3: 20 µg of rat brain lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N388A-10_Purified_Ankyrin-R_Antibody_2_112018
    Western blot of purified anti-Ankyrin-R antibody (clone N388A/10). Lane 1: Molecular weight marker; Lane 2: 40 µg of human cerebellum lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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857701 25 µg £57
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857702 100 µg £113
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Description

Ankyrin-R is an anchor protein whose main role in vivo is to anchor integral membrane protein/complexes to the cytoskeleton. It was originally discovered in erythrocytes, but later to be found expressed in the brain. The gene encoding Ankyrin-R, ANK1, has a highly complex regulatory mechanism, which gives rise to a variety of different isoforms of Ankyrin-R. Recent studies in Alzheimer’s Disease have shown that epigenetic dysregulation of ANK1, in combination of several other proteins including Trem-2, could be a contributing factor of increased TNFA expression and neuroinflammation1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Taylor A, et al. 2017. Front Cell Neurosci. 11:11 (IHC-F)
  2. Saifetiarova J, et al. 2017. J Neurosci. 37(10):2524 (IHC-F)
  3. Taylor A, et al. 2018. eNeuro. 5(3) (IHC-F, WB)
RRID
AB_2783477 (BioLegend Cat. No. 857701)
AB_2783477 (BioLegend Cat. No. 857702)

Antigen Details

Structure
ANK1 is a 1881 amino acid protein with an apparent molecular mass of ~ 206 kD.
Distribution

Tissue Distribution: Brain and bone marrow.
Cellular Distribution: Plasma membrane, cytoskeleton, cytosol, nucleus, and endoplasmic reticulum.

Function
ANK1 functions as an adaptor protein to anchor integral membrane proteins/complexes to the cytoskeleton.
Interaction
SPTB, SLC4A1, EPB41, TTN, OBSCN, HIF1AN
Biology Area
Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adaptor Proteins
Antigen References
  1. Morris G, et al. 2018. Mol Neurobiol. doi: 10.1007/s12035-018-1092-y.
  2. Nicolas V, et al. 2006. Transfuse Clin Biol. 13(1-2):23
  3. Kline CF, et al. 2006. Expert Rev Cardiovasc Ther. 4(4):477
Gene ID
286 View all products for this Gene ID 11733 View all products for this Gene ID 306570 View all products for this Gene ID
UniProt
View information about Ankyrin-R on UniProt.org
Go To Top Version: 1    Revision Date: 11/21/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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