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Purified anti-APCS (PTX2) Antibody

Pricing & Availability
Clone
W19147A (See other available formats)
Regulatory Status
RUO
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A. W19147A_PURE_APCS_IHC-P_032023
IHC staining of purified anti-APCS (PTX2) (clone W19147A) on formalin-fixed paraffin-embedded human testis tissue. Following antigen retrieval using Tris-EDTA, pH 9.0, the tissue was incubated without (panel A) and with (panel B) 5.0 µg/mL of primary antibody followed by incubation with Alexa Fluor® 647 goat anti-rat IgG (magenta) (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801). Images were captured with a 10X objective. Scale bar: 50 µm
  • A. W19147A_PURE_APCS_IHC-P_032023
    IHC staining of purified anti-APCS (PTX2) (clone W19147A) on formalin-fixed paraffin-embedded human testis tissue. Following antigen retrieval using Tris-EDTA, pH 9.0, the tissue was incubated without (panel A) and with (panel B) 5.0 µg/mL of primary antibody followed by incubation with Alexa Fluor® 647 goat anti-rat IgG (magenta) (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801). Images were captured with a 10X objective. Scale bar: 50 µm
  • W19147A_PURE_APCS_WB_032023
    Whole cell extracts (15 µg total protein) from NTERA-2 cells, THP-1 cells spiked with recombinant human APCS protein (Cat. No. 556606), and THP-1 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL of purified anti-APCS (PTX2) (clone W19147A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control at a 1:10000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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614651 25 µg £83
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614652 100 µg £271
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Description

APCS (amyloid P component, serum), also known as pentraxin 2 (PTX2), is a member of the pentraxin family, which has a non-covalently linked pentameric organization. This protein is characterized by its calcium-dependent ligand binding and distinctive flattened β-jellyroll structure similar to legume lectins. Human APCS shares a 79% amino acid homology with its mouse counterpart. APCS is secreted by hepatocytes as part of the acute phase protein synthesis in response to infections. Upon binding to its ligands, APCS is involved in the activation of classical complement cascade. In a mouse model, APCS was shown to downregulate acute inflammation accompanying experimental autoimmune encephalomyelitis by regulating T cell activity. In addition to its role in regulating immune response, APCS is found to be a component of amyloid deposits which have the ability of stabilizing amyloid fibrils and protecting them from proteolytic degradation. It is postulated that APCS may play a role in Alzheimer's disease and other amyloidosis. Interestingly, APCS is also identified as a component of basement membrane proteins and is able to bind laminin, collagen IV, P-selectin, and fibronectin in a Ca2+ dependent manner. In addition, APCS is known to specifically localize in human atherosclerotic lesions, which suggests that it may play a role in atherogenesis. Functional studies revealed that APCS binds to lipoproteins, specifically to HDL, but not LDL.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human APCS
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2941618 (BioLegend Cat. No. 614651)
AB_2941618 (BioLegend Cat. No. 614652)

Antigen Details

Structure
Monomer
Distribution

Liver, serum and urine

Function
APCS recognizes microbial pathogens and activates the classical complement pathway; it binds to Fcγ receptors and opsonizes particles for phagocytosis
Interaction
Cells expressing Fcγ receptors
Ligand/Receptor
FcγR (CD16, CD32, and CD64), collagen IV, laminin, HDL, VLDL, fibronectin, CRP, and CD62L
Biology Area
Complement, Neurodegeneration, Neuroinflammation
Molecular Family
APP/β-Amyloid, APP/Aβ Degradation
Antigen References
  1. Tennent GA, et al. 1995. Proc Natl Acad Sci USA. 92:4299-303.
  2. Li XA, et al. 1998. Biochem Biophys Res Commun. 244:249-52.
  3. Zahedi K. 1996. J Biol Chem. 271:14897-902.
  4. Pepys M, et al. 2002. Nature. 417:254-9.
  5. Stibenz D, et al. 2006. Eur J Immunol. 36:446-56.
  6. Lu J, et al. 2008. Nature. 456:989-92.
Gene ID
325 View all products for this Gene ID
UniProt
View information about APCS on UniProt.org
Go To Top Version: 1    Revision Date: 03/30/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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