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Purified anti-APEX1 Antibody

Pricing & Availability
Clone
1518CT337.123.86.269.232 (See other available formats)
Regulatory Status
RUO
Other Names
Apurinic/Apyrimidinic Endodeoxyribonuclease 1, REF1, HAP1, APEN, APE, APX, AP Endonuclease 1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1518CT33712386269232_PURE_APEX1_1_Antibody_011222
Whole cell extracts (15 µg total protein) from the indicated cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL of purified anti-APEX1 (clone 1518CT337.123.86.269.232) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control at a 1:10000 dilution (lower). Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • 1518CT33712386269232_PURE_APEX1_1_Antibody_011222
    Whole cell extracts (15 µg total protein) from the indicated cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL of purified anti-APEX1 (clone 1518CT337.123.86.269.232) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control at a 1:10000 dilution (lower). Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • 1518CT33712386269232_PURE_APEX1_Antibody_2_2_011222
    IHC staining of purified anti-APEX1 (clone 1518CT337.123.86.269.232) on formalin-fixed paraffin-embedded colon tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5.0 μg/mL of purified mouse IgG1 isotype control (Cat. No. 400102) (panel A) or 5.0 μg/mL purified anti-APEX1 (panel B) overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB, Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The images were captured with a 20X objective. Scale bar: 50 μm
  • 1518CT33712386269232_PURE_APEX1_Antibody_3_3_011222
    ICC staining of purified anti-APEX1 (clone 1518CT337.123.86.269.232) on HeLa cells. The cells were fixed and permeabilized with 100% methanol, and blocked with 5% FBS for 1 hour. The cells were then stained with 10 µg/mL of purified mouse IgG1, κ isotype control (Cat. No. 400102) (panel A) or 10 µg/mL of anti-APEX1 antibody (panel B), followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 anti-goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI, and the images were captured with a 40X objective. Scale bar: 50 μm
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605051 25 µg £81
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605052 100 µg £201
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Description

Apurinic-apyridimic endonuclease-1, or APEX1, is a multifunctional enzyme required for the repair of DNA damaged by both endogenous and exogenous agents. It also exerts oncogenic function as a redox signaling protein by reductively stimulating the DNA-binding activity of transcription factors that drive cancer progression, including c-Jun, AP-1, NF-κB, p53, and HIF1α. APEX1 expression level and subcellular localization are positively correlated with cancer progression, and elevated APEX1 expression may also confer resistance to DNA-damaging chemotherapeutic agents.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length recombinant human APEX1 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration of 5.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

When using this clone for ICC, we recommend using PFA + methanol or methanol only fix-perm conditions. We do not recommend using PFA + Triton X-100 due to non-specific staining.

When testing this clone for ICC, this antibody produced filamentous, extranuclear staining in some cells. This pattern was also observed with a control antibody generated using a different APEX1 immunogen, consistent with this staining being APEX1 dependent.

RRID
AB_2910484 (BioLegend Cat. No. 605051)
AB_2910484 (BioLegend Cat. No. 605052)

Antigen Details

Structure
APEX1 is a 318 amino acid protein with a predicted molecular weight of ~36 kD.
Distribution

Nucleus/Ubiquitously expressed

Function
DNA repair enzyme
Biology Area
Cell Biology, DNA Repair/Replication
Molecular Family
Enzymes and Regulators
Antigen References
  1. Fishel ML, et al. 2008. DNA Repair. 7:177-86.
  2. Kim MH, et al. 2013. J Clin Invest. 123:3211-3230.
  3. Thakur, et al. 2014. Exp Mol Med. 46:e106.

 

Gene ID
328 View all products for this Gene ID
UniProt
View information about APEX1 on UniProt.org

Other Formats

View All APEX1 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-APEX1 1518CT337.123.86.269.232 WB,ICC,IHC-P
Go To Top Version: 1    Revision Date: 01/12/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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