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Purified anti-Apo E Antibody

Pricing & Availability
Clone
3B3C32 (See other available formats)
Regulatory Status
RUO
Other Names
Apolipoprotein E, ApoE
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
3B3C32_Purified_Apo-E_Antibody_WB_090916
Total cell lysates (15 µg protein) from U251, mouse liver, mouse brain and 3T3-L1 were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with anti-Apo-E Antibody (clone 3B3C32). Proteins were visualized using a goat anti-rat IgG secondary antibody conjugated to HRP and chemiluminescence detection. Purified anti-β-actin antibody was used as a loading control.
  • 3B3C32_Purified_Apo-E_Antibody_WB_090916
    Total cell lysates (15 µg protein) from U251, mouse liver, mouse brain and 3T3-L1 were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with anti-Apo-E Antibody (clone 3B3C32). Proteins were visualized using a goat anti-rat IgG secondary antibody conjugated to HRP and chemiluminescence detection. Purified anti-β-actin antibody was used as a loading control.
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674802 100 µg £182
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Description

Apo-E is a class of apolipoprotein primarily synthesized in the liver, but is also found in other tissues such as brain, kidneys, and spleen. Apo-E is a protein component of serum chylomicron and lipoprotein particles, playing essential roles in cholesterol transportation, homeostasis, and metabolism. The expression of Apo-E is induced by cholesterol-rich diets and hyperlipoproteinemia. In the central nervous system, Apo-E is produced by astrocytes and microglia. The Apo-E-containing lipoprotein particles play important functions in brain injury repair, neurogenesis, and synaptic plasticity. Apo-E has been found to be involved in the regulation of amyloid-β aggregation and clearance. Genetic polymorphisms of Apo-E are associated with neuron degenerative disorders including Alzheimer's disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Full length mouse Apo-E recombinant protein expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Moon HJ, et al. 2022. Neurobiol Dis. 164:105631. PubMed
  2. Jeong A, et al. 2021. Acta Neuropathol Commun. 9:129. PubMed
RRID
AB_2565426 (BioLegend Cat. No. 674802)

Antigen Details

Structure
293 amino acids, predicted molecular weight of 34 kD (mature form), and contains one LDL receptor binding domain and two heparin binding regions.
Function
Apo-E is a component of serum chylomicron, VLDL, and HDL particles, and mediates lipid metabolism and memory formation.
Interaction
SET, KCNMA1, SAA, and PRNP.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Antigen References

1. Hayes EM, et al. 2014. Front Immunol. 5:537.
2. Kim J, et al. 2014. Mol. Cells 37:767.
3. Verghese PB, et al. 2013. Proc. Natl. Acad. Sci. USA 110:E1807.
4. Mahley RW, et al. 2012. Neuron 76:871.
5. Holtzman DM, et al. 2012. Cold Spring Harb. Perspect. Med. 2:a006312.
6. Zhang HL, et al. 2010. Clin. Dev. Immunol. 2010:186813.

Gene ID
11816 View all products for this Gene ID
UniProt
View information about Apo E on UniProt.org
Go To Top Version: 2    Revision Date: 09/09/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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