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Purified anti-Apo E4 Antibody (Previously Covance catalog# MMS-5016)

Pricing & Availability
Clone
5A9 (See other available formats)
Regulatory Status
RUO
Other Names
APO-E, Apolipoprotein E, Apolipoprotein E4, ApoE
Previously
Covance Catalog# MMS-5016
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
5A9_PURE_ApoE4_WB_113018
Western blot of purified anti-Apo E4 antibody (clone 5A9). Lane 1: Molecular weight marker; Lane 2: 20 µg of human plasma; Lane 3: 0.05 µg of APO E4 peptide; Lane 4: 0.05 µg of APO E3 peptide; Lane 5: 0.05 µg of APO E2 peptide. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 5A9_PURE_ApoE4_WB_113018
    Western blot of purified anti-Apo E4 antibody (clone 5A9). Lane 1: Molecular weight marker; Lane 2: 20 µg of human plasma; Lane 3: 0.05 µg of APO E4 peptide; Lane 4: 0.05 µg of APO E3 peptide; Lane 5: 0.05 µg of APO E2 peptide. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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811701 100 µL £189
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Description

Apolipoprotein E (ApoE) belongs to a class of apolipoproteins that binds to lipids to form lipoproteins. ApoE is produced by the liver and macrophages in peripheral tissues where it regulates cholesterol metabolism. In the central nervous system, ApoE is primarily produced by astrocytes and plays an important role in transporting cholesterol to neurons via ApoE receptors. ApoE has three major isoforms corresponding to three alleles (ApoE2, ApoE3 and ApoE4) that differ from each other by only one or two amino acids at positions 112 and 158. ApoE4 is one of the largest known genetic risk factors for late-onset Alzheimer's disease. Further, ApoE4 has been implicated in several other diseases such as atherosclerosis, impaired cognitive function and multiple sclerosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a peptide sequence derived from the human APO E4 protein.
Formulation
Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2564760 (BioLegend Cat. No. 811701)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family
Apolipoproteins
Gene ID
348 View all products for this Gene ID
UniProt
View information about Apo E4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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