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Purified anti-Arginase 1 Antibody

Pricing & Availability
Clone
O94E6/ARG1 (See other available formats)
Regulatory Status
RUO
Other Names
Arginase 1, liver type Arginase, Type I Arginase, ARG1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A-O94E6-ARG1_PURE_Arginase-1_Antibody_WB_071519
Whole cell extracts (15 µg protein) from HeLa cells transfected with non-targeting control siRNA (siCON) or siRNA targeting Arginase 1 (siARG1) were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 0.5 µg/mL (1:1000 dilution) of Purified anti-Arginase 1 Antibody, clone O94E6/ARG1, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 643807) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • A-O94E6-ARG1_PURE_Arginase-1_Antibody_WB_071519
    Whole cell extracts (15 µg protein) from HeLa cells transfected with non-targeting control siRNA (siCON) or siRNA targeting Arginase 1 (siARG1) were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 0.5 µg/mL (1:1000 dilution) of Purified anti-Arginase 1 Antibody, clone O94E6/ARG1, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 643807) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • B-O94E6slashARG1_PURE_Arginase-1_Antibody_WB_011718
    Total lysates (15 µg protein) from Jurkat, MCF-7 and HepG2 were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1 µg/ml) purified anti-Arginase-1 antibody, clone O94E6/ARG1. Proteins were visualized using chemiluminescence detection by incubation with HRP Goat anti-mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-GAPDH was used as a loading control (Cat. No. 649203, 1:1000 dilution).
  • C-O94E6_ARG1_PURE_Arginase1_Antibody_052022
    IHC staining using purified anti-Arginase 1 antibody (clone O94E6/ARG1) on formalin-fixed paraffin-embedded human liver tissue. The tissue was incubated with 10 μg/mL of anti-Arginase 1 antibody overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 goat anti-mouse IgG antibody (red) (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scalebar = 50 μM.
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678801 25 µg £70
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678802 100 µg £174
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Description

ARG1 (Arginase 1), is a marker for the M2 anti-inflammatory macrophage subset that hydrolyzes l-arginine into urea and ornithine, and plays an important role in wound healing, antiviral immune responses, and atherosclerosis. The inherited deficiency of this enzyme results in argininemia, which is an autosomal recessive disorder characterized by hyperammonemia. Recent studies show that intracellular pathogens induce the expression of ARG1 in mouse macrophages through the Toll-like receptor (TLR) pathway. The deletion of this enzyme in macrophages favors host survival. Other studies indicate that ARG1 also plays a role in T cell responses and selective antagonists of ARG1 might prove beneficial in fighting diseases in which T-cell responses are inappropriately suppressed.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant human ARG1 protein produced in HEK293T cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per mL. For immunohistochemistry, a concentration range of 1 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Altemus J, et al. 2022. J Cell Physiol. 237:3001. PubMed
  2. Fitterer R, et al. 2020. J Clin Med. 9:00. PubMed
  3. Joseph R, et al. 2021. Br J Cancer. 125:176. PubMed
  4. Paredes F, et al. 2020. Circ Res. 126:41:00. PubMed
  5. Zhang X, et al. 2020. Arterioscler Thromb Vasc Biol. 40:597. PubMed
RRID
AB_2566050 (BioLegend Cat. No. 678801)
AB_2566050 (BioLegend Cat. No. 678802)

Antigen Details

Structure
322 amino acids with a predicted molecular weight of approximately 35 kD.
Distribution

Expressed predominantly in the liver. It can also be induced in macrophages. Cytoplasmic.

Function
Arginase 1 plays an important role in processes such as wound healing, antiviral immune response, and atherosclerosis.
Interaction
Binds 2 manganese ions per subunit as a cofactor.
Ligand/Receptor
l-arginine.
Cell Type
Dendritic cells, Macrophages
Biology Area
Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
Enzymes and Regulators
Antigen References

1. Wu G, et al. 1998. Biochem. J. 336:1.
2. El Kasmi KC, et al. 2008. Nat. Immunol. 9:1399.
3. Pesce JT, et al. 2009. PLoS Pathog. 5:e1000371.
4. Burrack KS, et al. 2014. Front Immunol. 5:428.
5. Pourcet B, et al. 2013. Trends Cardiovasc. Med. 23:143.
6. Bronte V, et al. 2003. Trends Immunol. 24:302.

Gene ID
383 View all products for this Gene ID
UniProt
View information about Arginase 1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 05/20/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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