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Purified anti-Aspartate Aminotransferase Antibody

Pricing & Availability
Clone
Poly28616 (See other available formats)
Regulatory Status
RUO
Other Names
GOT1, Aspartate Aminotransferase
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly28616_Purified_Aspartate-Aminotransferase_Antibody_1_050119
Western blot of purified anti-Aspartate Aminotransferase antibody (Poly28616). Lane 1: Molecular weight marker; Lane 2: 20 µg of human cerebellum lysate. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled Donkey anti-Rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28616_Purified_Aspartate-Aminotransferase_Antibody_1_050119
    Western blot of purified anti-Aspartate Aminotransferase antibody (Poly28616). Lane 1: Molecular weight marker; Lane 2: 20 µg of human cerebellum lysate. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled Donkey anti-Rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28616_Purified_Aspartate-Aminotransferase_Antibody_2_050119
    IHC staining of purified anti-Aspartate Aminotransferase antibody (Poly28616) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
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861601 25 µg £86
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Description

GOT1 is the cytoplasmic form of aspartate aminotransferase and plays a role in amino acid metabolism, and the tricarboxylic acid cycles. GOT1 is one of the 6 genes that is commonly shared and regulated in the aging population and AD patients, suggesting that it may contribute as an aging-dependent AD risk factor1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Recombinant Aspartate Aminotransferase (AST)
Formulation
0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 0.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2810746 (BioLegend Cat. No. 861601)

Antigen Details

Structure
GOT1 is a 413 amino acid protein with an apparent molecular mass of ~ 46 kD.
Distribution

Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Cytosol and nucleus

Function
GOT1 is a cytoplasmic aspartate aminotransferase that plays a role in amino acid metabolism and the tricarboxylic acid cycles.
Interaction
Forms homodimer.
Cell Type
Astrocytes, Mature Neurons, Microglia, Neural Stem Cells, Neurons, Oligodendrocytes
Biology Area
Neurodegeneration, Neuroinflammation, Neuroscience
Antigen References
  1. Zheng JJ, et al. 2018. Medicine (Baltimore). 97(28):e11343
  2. Zhang K, et al. 2018. Mol Carcinog. 57(11):1566
Gene ID
2805 View all products for this Gene ID
UniProt
View information about Aspartate Aminotransferase on UniProt.org
Go To Top Version: 1    Revision Date: 05/01/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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