- Clone
- W21106A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- activating transcription factor 2 formerly, ATF2, cAMP-responsive element-binding protein 2, CREB2, CREBP1, CRE-BP1, cyclic AMP-dependent transcription factor ATF-2, HB16, MGC111558, TREB7
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
630051 | 25 µg | £141 | ||||
630052 | 100 µg | £339 |
The Activating Transcription Factor 2 (ATF-2), also known as CREB2 and CREBP1, contains a Basic Leucine Zipper (bZIP) domain and a C2H2-type zinc finger domain. ATF-2 binds to both DNA response factors AP-1 and CRE and belongs to the ATF/CREB family of leucine zipper proteins. ATF-2 interacts with a variety of viral oncoproteins and cytosolic tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways. ATF-2 activity can be initiated by various forms of cellular stress. Several studies have shown aberrant ATF-2 activation in the development and progression of mammalian skin tumors. ATF-2 may mediate carcinogenesis induced by the mutant Ras protein and regulate the maintenance of the invasive cancer phenotype of several epithelial cell types. ATF-2 has also been shown to be phosphorylated at its C-terminus (serine 472 and 480 in mice, serine 490 and 498 in human) by ATM during double-strand breaks. Mice with mutations of these two serines are sensitive to irradiation and more prone to tumorigenesis under p53 knockout condition.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial Recombinant human ATF2 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 2.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
The clone cross-reacts with mouse in western blot (WB).
For immunocytochemistry (ICC), 4% PFA fixation followed by permeabilization with 0.5% Triton X-100 is recommended.
This product is not recommended for use in immunohistochemistry (IHC). - Additional Product Notes
-
This antibody has been tested in knockout/knockdown models for Western Blotting and Immunocytochemistry.
- RRID
-
AB_3097336 (BioLegend Cat. No. 630051)
AB_3097336 (BioLegend Cat. No. 630052)
Antigen Details
- Structure
- ATF-2 protein is a 505 amino acid protein with a predicted molecular weight of 55 kD
- Distribution
-
Abundant expression in the brain
- Function
- Transcriptional activator
- Interaction
- SMAD3, SMAD4, UTF1, NBN, MRE11, XPO1, KAT5, CUL3
- Biology Area
- Cell Biology, DNA Repair/Replication, Transcription Factors
- Antigen References
-
- Abdel-Hafiz, H.A. et al. 1992. Mol Endocrinol. 6:2079-89.
- Gupta S, et al. 1995. Science. 267:389-93.
- Van Dam H, et al. 1995. EMBO J. 14:1798-811.
- Livingstone C, et al. 1995. EMBO J. 14:1785-97.
- Yamasaki T, et al. 2009. J Cell Biochem. 284:8567-81.
- Leslie MC, et al. 2009. J Cell Biochem. 94:25–38.
- Papassava P, et al. 2004. Cancer Res. 64:8573–84.
- Vlahopoulos SA, et al. 2008. BioEssays. 30:314–27.
- Bhoumik A, et al. 2005. Mol.Cell. 18:577–87.
- Li S, et al. 2010. Genes Cancer. 1:316–330.
- Gene ID
- 1386 View all products for this Gene ID
- UniProt
- View information about ATF-2 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All ATF-2 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-ATF-2 | W21106A | WB,ICC |
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