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Purified anti-BACH2 Antibody

Pricing & Availability
Clone
16B10B53 (See other available formats)
Regulatory Status
RUO
Other Names
BTB and CNC homolog 2, Transcription regulator protein BACH2,
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
16B10B53_PURE_BACH2_Antibody_WB_031317
Total cell lysates (15µg protein) from THP1 and Raji (negative controls) and Ramos were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1µg/mL purified anti-BACH2 antibody (clone 16B10B53) antibody (upper). Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M*: MW ladder with longer exposure time.
  • 16B10B53_PURE_BACH2_Antibody_WB_031317
    Total cell lysates (15µg protein) from THP1 and Raji (negative controls) and Ramos were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1µg/mL purified anti-BACH2 antibody (clone 16B10B53) antibody (upper). Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M*: MW ladder with longer exposure time.
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695602 100 µg £189
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Description

BTB and CNC Homolog 2 (BACH2) is a transcription factor of the basic leucine zipper family. BACH2 participates in the regulation of both innate and adaptive immune responses. In B cells, BACH2 plays critical roles in somatic hypermutation, class-switch recombination, memory B cell formation, and repression of myeloid genes. In T cells, BACH2 promotes the development of Foxp3+ regulatory T cells and memory T cells. Bach2 is required for the maintenance of naive CD4 T cells by suppressing effector T cell differentiation. BACH2 is also required for the maturation and function of alveolar macrophages. Defects in BACH2 gene are associated with autoimmune and allergic diseases, such as asthma, type 1 diabetes, celiac disease, rheumatoid arthritis, and generalized vitiligo.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human BACH2 recombinant protein (513-841 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2650879 (BioLegend Cat. No. 695602)

Antigen Details

Structure
841 amino acids with a predicted molecular weight of 92.5 kD. Contains a N-terminal BTB/POZ domain for protein-protein interaction and a C-terminal bZIP domain for DNA binding.
Distribution

Nucleus and cytoplasm.

Function
BACH2 is a transcription factor involved in the development and differentiation of both B and T cells.
Interaction
Forms homodimers or heterodimers with Maf-related transcription factors.
Cell Type
B cells, Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Kuwahara M, et al. 2016. Nat. Commun. 7:12596.
2. Sidwell T, et al. 2016. Nat. Immunol. 17:744.
3. Roychoudhuri R, et al. 2016. Nat. Immunol. 17:851.
4. Itoh-Nakadai A, et al. 2014. Nat. Immunol. 15:1171.
5. Kuwahara M, et al. 2014. Nat. Commun. 5:3555.
6. Kim EH, et al. 2014. J. Immunol. 192:985.

Gene ID
60468 View all products for this Gene ID
UniProt
View information about BACH2 on UniProt.org
Go To Top Version: 0    Revision Date: 03/15/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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