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Purified anti-Bax Antibody

Pricing & Availability
Clone
6A7 (See other available formats)
Regulatory Status
RUO
Other Names
BCL2 associated X protein, apoptosis regulator Bax
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
6A7_BAX_Purified_Antibody_WB_012114.jpg
Immunoprecipitation/Western blot analysis of extracts from Daudi cells(lane 1 and lane 2) and THP-1 cells (lane 4 and lane5). Lane 4 contains THP-1 lysate input (5%). Lane 1 and lane 4 were immunoprecipitated with mouse IgG1 isotype control antibodies and lane 2 and lane 5 were immunoprecipitated with BAX antibody (6A7). Western blot analysis was performed using rabbit anti-BAX antibody (poly6251).
  • 6A7_BAX_Purified_Antibody_WB_012114.jpg
    Immunoprecipitation/Western blot analysis of extracts from Daudi cells(lane 1 and lane 2) and THP-1 cells (lane 4 and lane5). Lane 4 contains THP-1 lysate input (5%). Lane 1 and lane 4 were immunoprecipitated with mouse IgG1 isotype control antibodies and lane 2 and lane 5 were immunoprecipitated with BAX antibody (6A7). Western blot analysis was performed using rabbit anti-BAX antibody (poly6251).
Cat # Size Price Quantity Check Availability Save
633801 25 µg £70
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633802 100 µg £152
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Description

Bax is a 21 kD pro-apoptotic protein known to regulate apoptosis. Bax is found in the cytoplasm, mitochondria, and nucleus and is highly expressed in hematopoietic stem cells, the ovary, and in the lymph node. Bax binds the anti-apoptotic protein Bcl-2 as a heterodimer or forms homodimers. The relative levels of pro-apoptotic proteins such as Bax and anti-apoptotic proteins such as Bcl-2 determines whether cell death will occur following an apoptotic stimulus. Bax accelerates the opening of mitochondrial VDAC altering membrane potential and allowing cytochrome c to pass out of the mitochondria into the cytosol to initiate downstream caspase activation. p53 can transcriptionally activate the Bax gene to induce apoptosis. Bax has been shown to be mutated in some human cancers. Clone 2D2 has been shown to be useful for western blotting, immunoprecipitation, immunofluorescence and immunohistochemistry of the human Bax protein.This antibody does not cross-react with Bcl-2 or Bcl-XL proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, Monkey
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Amino acid:12-24 common to human, mouse, and rat Bax protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted at between 2°C and 8°C.
Application

IP - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunoprecipitation. This antibody can be used at 2-4 µg /1 x107 cell equivalents for immunoprecipitation. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Hsu YT, et al. 1997. J. Biol. Chem. 272:13829.
  2. Liu G, et al. 2013. J. Immunol. 191:2680. PubMed
Product Citations
  1. Oda SK, et al. 2020. J Exp Med. 217: . PubMed
  2. Liu G, et al. 2013. J Immunol. 191:2680. PubMed
RRID
AB_2061543 (BioLegend Cat. No. 633801)
AB_2061543 (BioLegend Cat. No. 633802)

Antigen Details

Structure
Forms homodimers and heterodimers with Bcl-2, approximately 21 kD
Distribution

Cytoplasm, mitochondria, nucleus. Expressed in hematopoietic stem cells, ovary, lymph node

Function
Induces cell death through the process of apoptosis by acting on mitochondria.
Interaction
14-3-3 theta, VDAC1, Bcl-2, solute carrier family 25, mitochondrial carrier member 4
Cell Type
Hematopoietic stem and progenitors
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience
Antigen References

1. LeBlanc H, et al. 2002. Nat. Med. 8:274.
2. Marzo I, et al. 1998. Science 281:2027.
3. Miyashita T et al. 1995. Cell 80:293.
4. Oltvai ZN, et al. 1993. Cell 74:609.

Gene ID
581 View all products for this Gene ID
UniProt
View information about Bax on UniProt.org
Go To Top Version: 2    Revision Date: 01/21/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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