- Clone
- W18024A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Branched Chain Amino Acid Transanimase 1, BCT1, BCATC, PNAS121, Protein ECA39
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
938001 | 25 µg | £70 | ||||
938002 | 100 µg | £174 |
BCAT1 is an aminotransferase that catalyzes the catabolism of the branched-chain amino acids leucine, isoleucine, and valine, making it an important regulator of the TCA cycle. BCAT1 also plays a role in cell cycle progression, differentiation, and apoptosis. Clinical disorders involved with mutations in the BCAT1 gene include hypervalinemia and hyperleucine-isoleucinemia.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Full-length recombinant human BCAT1 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This clone failed to immunoprecipitate BCAT1 from NTERA-2 lysates.
This clone was tested for immunocytochemistry on NTERA-2 cells fixed with 4% PFA and permeabilized with either methanol or Triton X-100. Both methods resulted in dim staining of BCAT1. We therefore do not recommend the clone for this application. - RRID
-
AB_2876757 (BioLegend Cat. No. 938001)
AB_2876757 (BioLegend Cat. No. 938002)
Antigen Details
- Structure
- BCAT1 is a 386 amino acid protein with a predicted molecular weight of 43 kD.
- Distribution
-
Ubiquitously expressed/Cytosolic
- Function
- Aminotransferase enzyme, amino acid catabolism
- Cell Type
- Macrophages
- Biology Area
- Cell Biology, Mitochondrial Function
- Molecular Family
- Enzymes and Regulators
- Antigen References
-
- Papathanassiu AE, et al. 2017. Nat. Commun. 8:16040.
- Xu M, et al. 2016. Oncol Lett. 12:2648-2656.
- Zhou W, et al. 2013. Mol Cancer. 12:53.
- Wegermann K, et al. 2018. PLoS One. 13:e0204308.
- Hattori A, et al. 2017. Nature. 545:500-504.
- Gene ID
- 586 View all products for this Gene ID
- UniProt
- View information about BCAT1 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All BCAT1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-BCAT1 Antibody | W18024A | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-BCAT1 Antibody
Whole cell lysates (15 µg) from NTERA-2, Daudi, and indicate...
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