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Purified anti-BCL10 Antibody

Pricing & Availability
Clone
P84F8 (See other available formats)
Regulatory Status
RUO
Other Names
B-Cell CLL/Lymphoma 10, B-Cell Lymphoma/Leukemia 10, Bcl-10, BCL-10, CARD Containing Molecule Enhancing NF-KB, CLAP, Me10, CIPER, c-E10, CARMEN, IMD37
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P84F8_PURE_BCL10_Antibody_WB_0370618
Total cell lysates (15 µg protein) from NIH3T3 (lane 1), HeLa (lane 2) and HaCaT (lane 3) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 0.5 µg/mL (1:1000 dilution) of purified anti-BCL10 antibody (clone P84F8) (upper blot). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for anti-BCL10 antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin antibody, clone 2F1-1(lower blot). Lane M: Molecular weight ladder.
  • P84F8_PURE_BCL10_Antibody_WB_0370618
    Total cell lysates (15 µg protein) from NIH3T3 (lane 1), HeLa (lane 2) and HaCaT (lane 3) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 0.5 µg/mL (1:1000 dilution) of purified anti-BCL10 antibody (clone P84F8) (upper blot). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for anti-BCL10 antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin antibody, clone 2F1-1(lower blot). Lane M: Molecular weight ladder.
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684702 100 µg £182
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Description

BCL10 (B cell lymphoma 10), together with CARMA1 (CARD11) and lymphoid tissue lymphoma-translocation gene 1 (MALT1) are signaling proteins. They have a key role in antigen-receptor-mediated lymphocyte activation through the NF-κB pathway. Activation of NF-κB is essential for the immune system to function normally. Deregulated NF-κB in lymphocytes can lead to immunodeficiency, but also to autoimmunity or lymphomas. Phosphorylation of the CARMA1 and BCL10 proteins and ubiquitylation of BCL10 affect the formation and stability of the CARMA1-BCL10-MALT1 (CBM) complex, and also control negative feedback regulation of the NF-κB signaling pathway. Also, recent studies point out an unexpected role for BCL10 in the regulation of the actin cytoskeleton.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant fragment of human BCL10 expressed in E. Coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Reacts with mouse BCL10.

RRID
AB_2572170 (BioLegend Cat. No. 684702)

Antigen Details

Structure
233 amino acids with the predicted molecular weight of approximately 26kD with the characteristic of caspase recruitment domain.
Distribution

BCL10 is expressed in all normal and malignant tissues. In the lymphoid tissue, it is highly expressed in the germinal center but low in the mantle zone, and intermediate in the marginal zone.

Function
Antigen-receptor-mediated lymphocyte activation through the NF-κB pathway.
Interaction
CARMA1, CARD9, 10 and 14, MALT1, BCR, TCR
Cell Type
B cells, Neutrophils
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Signal Transduction, Transcription Factors, Ubiquitin/Protein Degradation
Antigen References

1. Turvey SE, et al. 2014. J. Allergy Clin. Immunol. 134:276.
2. Thome M, et al. 2010. Cold Spring Harb. Perspect. Biol. 2:a003004.
3. Thome M, et al. 2007. Trends Immunol. 28:281.
4. Pérez de Diego R, et al. 2015. J. Allergy Clin. Immunol. 136:1139.
5. Sun W, et al. 2010. Cell Signal. 22:1799.
6. Jost P, et al. 2006. Curr. Drug Targets 7:1335.
7. Lin X, et al. 2004. Semin. Immunol. 16:429.
8. Thome M, et al. 2004. Nat. Rev. Immunol. 4:348.
9. Marion S, et al. 2012. Dev. Cell 5:954-967.

Gene ID
8915 View all products for this Gene ID
UniProt
View information about BCL10 on UniProt.org
Go To Top Version: 2    Revision Date: 07/06/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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