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Purified anti-β-actin Antibody

Pricing & Availability
Clone
Poly6221 (See other available formats)
Regulatory Status
RUO
Other Names
Actin, cytoplasmic 1
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
poly6221_PURE_betaactin_Antibody_1_WB_090616
Total cell lysate from HeLa cells (lane 1, 15 µg), NIN3T3 cells (lane 2, 15 µg), U87-MG cells (lane 3, 15 µg), mouse brain (lane 4, 15 µg) and rat brain (lane 5, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-β-actin antibody (Poly6221). Proteins were visualized using an HRP Donkey anti-rabbit IgG Antibody and chemiluminescence detection.
  • poly6221_PURE_betaactin_Antibody_1_WB_090616
    Total cell lysate from HeLa cells (lane 1, 15 µg), NIN3T3 cells (lane 2, 15 µg), U87-MG cells (lane 3, 15 µg), mouse brain (lane 4, 15 µg) and rat brain (lane 5, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-β-actin antibody (Poly6221). Proteins were visualized using an HRP Donkey anti-rabbit IgG Antibody and chemiluminescence detection.
  • poly6221_PURE_betaactin_Antibody_2_IF_062016
    Immunofluorescent microscope analysis of Hela cells using Poly6221, followed by Rhodamine Red-X goat anti-rabbit IgG and DAPI.
  • poly6221_PURE_betaactin_Antibody_3_IHCP_102720
    Formalin-fixed paraffin-embedded human kidney tissue was stained with Poly6221 and developed with an alkaline phosphatase chromogen substrate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
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622101 50 µL £81
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622102 200 µL £201
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Description

β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting). The Poly6221 antibody has been shown to be useful for Western blotting of mouse, rat, and human β-actin.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat, Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Peptide-KLH, NH2 terminus
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, store frozen at -20°C.
Application

WB - Quality tested
ICC, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µL per 5 mL antibody dilution buffer for each mini-gel. For immunocytochemistry: Use a dilution range of 1:100~1:400. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, use a 1:100 dilution of antibody for staining. Antigen retrieval for IHC using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product may contain other non-IgG subtypes.

Additional Product Notes
The 50 µL size can be used for approximately 5 Western blots, and the 200 µL size can be used for approximately 20 Western blots.
Application References

(PubMed link indicates BioLegend citation)
  1. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  2. Joyce CW. 2006. J. Biol. Chem. 281:33053.
  3. Yanagiya T,et al.2007.Obesity.15:572.PubMed
  4. KishidaT,et al.2007.J. Immunol. 179:8554.PubMed
  5. Ouimet M, et al. 2008. Arterisocler Thromb Vasc Biol. 28:1144. PubMed
  6. Toltlt LJ,et al. 2008.J. Immunol. 181:2165. PubMed
  7. Sawada. T,et al. 2008. J. Biol. Chem. 283:26820. PubMed
  8. Ikeda D, et al. 2008. Endocrinology. 149:6037. PubMed
  9. Rahman MK, et al. 2010. J. Immunol. 184:7247. PubMed
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  14. Harvey BS, et al. 2014. Cytokine. 65:236. PubMed
  15. Diessner J, et al. 2014. Cell Death Dis. 5:1149. PubMed
  16. Hua X, et al. 2015. PloS One. 10:128039. PubMed
Product Citations
  1. Checa M, et al. 2016. PLoS One. 11: 0150383. PubMed
  2. Okimoto T, et al. 2020. Cancer Sci. 111:1910. PubMed
  3. Nishina T, et al. 2023. iScience. 26:105934. PubMed
  4. Diessner J, et al. 2014. Cell Death Dis. 5:1149. PubMed
  5. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
  6. Mete B, et al. 2022. Life Sci Alliance. 5:. PubMed
  7. Al–Naggar IM, et al. 2019. J Gerontol A Biol Sci Med Sci. 74:325. PubMed
  8. Agabiti S, Li J, Wiemer A 2017. Cell Death Dis. 10.1038/cddis.2017.101. PubMed
  9. Wang D, et al. 2018. Immunity. 48:659. PubMed
  10. Piao X, et al. 2012. Sci Signal. 5:93. PubMed
  11. Wakatsuki S, et al. 2021. J Cell Biol. 220:. PubMed
  12. Harvey B, et al. 2014. Cytokine. 65:236. PubMed
  13. Wakatsuki S, et al. 2015. J Cell Biol. 211: 881 - 896. PubMed
  14. Kartika ID, et al. 2021. Cancer Med. 10:8988. PubMed
  15. Pfeil EM, et al. 2020. Molecular Cell. 80(6):940-954.e6. PubMed
  16. Hua X, et al. 2015. PLoS One. 10:128039. PubMed
  17. Nishina T, et al. 2021. Nat Commun. 12:2281. PubMed
  18. Takeda K, et al. 2017. Nat Commun. 8:14607. PubMed
  19. Che L, et al. 2019. Exp Ther Med. 18:1745. PubMed
  20. Ouimet M, et al. 2008. Arterioscler Thromb Vasc Biol. 28:1144. PubMed
  21. Rahman M, et al. 2010. J Immunol. 184:7247. PubMed
  22. Taha A, et al. 2020. J Cell Physiol. 7094:235. PubMed
  23. Nishigami Y, et al. 2016. Sci Rep. 6:18964. PubMed
  24. Man F, et al. 2021. Am J Physiol Lung Cell Mol Physiol. 321:L803. PubMed
  25. Toltl L, et al. 2008. J Immunol. 181:2165. PubMed
  26. Tao HC, et al. 2020. Front Immunol. 11:148. PubMed
  27. Berdasco C, et al. 2020. Front Cell Infect Microbiol. 0.681944444. PubMed
  28. Feddersen CR, et al. 2019. Cancer Res. 79:5074. PubMed
  29. Joyce C, et al. 2006. J Biol Chem. 281:33053. PubMed
  30. Zhu S et al. 2017. Cancer cell. 32(3):310-323 . PubMed
  31. Naneix F et al. 2017. eNeuro. 4(3) pii: ENEURO. PubMed
  32. Yanagiya T, et al. 2007. Obesity . 1.022222222. PubMed
  33. Kishida T, et al. 2007. J Immunol. 179:8554. PubMed
  34. Zheng X, et al. 2018. Int J Mol Med. 128:1300. PubMed
  35. Zhang Y, et al. 2012. Am J Physiol Renal Physiol. 302:F70. PubMed
  36. Terrazas M 2014. Int J Parasit. 44 613 . PubMed
  37. Caglar Beker M, et al. 2020. Turk J Med Sci. Online ahead of print. PubMed
  38. Ikeda D, et al. 2008. Endocrinology. 149:6037. PubMed
  39. Ng C, et al. 2013. J Virol. 87:9511. PubMed
  40. Fliegauf M, et al. 2021. Front Immunol. 12:621503. PubMed
  41. Grundmann M, et al. 2018. Nat Commun. 9:341. PubMed
  42. Pototskiy E, et al. 2021. Sci Rep. 11:17262. PubMed
  43. Sawada T, et al. 2008. J Biol Chem. 283:26820. PubMed
  44. Tsurusaki S, et al. 2019. Cell Death Dis. 10:449. PubMed
  45. Panwar P, et al. 2018. Br J Pharmacol. 175:902. PubMed
  46. Merten N et al. 2018. Cell chemical biology. 25(6):775-786 . PubMed
  47. Kumarasinghe N, et al. 2019. BMC Res Notes. 12:33. PubMed
  48. Inao T, et al. 2019. Cancer Sci. 110:2690. PubMed
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  50. Shikama Y, et al. 2011. Innate Immunity. 17:03. PubMed
RRID
SCR_001134 (BioLegend Cat. No. 622101)
SCR_001134 (BioLegend Cat. No. 622102)

Antigen Details

Structure
Member of the actin family; 42 KD
Distribution

Ubiquitously expressed in the cytoplasm of all eukaryotic cells

Function
Actins are highly conserved proteins that are involved in cell motility
Interaction
Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) that forms a two-stranded helix. Each actin can bind to four others.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Microfilaments
Antigen References

1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673.
2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133.
3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687.

Regulation
Three actin isoforms exist in vertebrates; alpha, found in muscle tissues; beta and gamma, found as cytoskeletal components and mediators of internal cell motility. Drugs such as cytochalasin disrupt the actin network.
Gene ID
11461 View all products for this Gene ID
UniProt
View information about beta-actin on UniProt.org
Go To Top Version: 6    Revision Date: 10/27/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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