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Purified anti-β-Amyloid, 1-16 Recombinant Antibody

Pricing & Availability
Clone
6E10.Rec (See other available formats)
Regulatory Status
RUO
Other Names
AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
6E10Rec_PURE_beta-amyloid_Antibody_1_052423.png
IHC staining of purified anti-β-Amyloid, 1-16 Recombinant (clone 6E10.Rec) on formalin-fixed paraffin-embedded human normal brain (panel A) or human Alzheimer's brain (panel B). Following antigen retrieval using 88% formic acid, the tissue was incubated without (-) or with (+) 5.0 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB, Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 6E10Rec_PURE_beta-amyloid_Antibody_1_052423.png
    IHC staining of purified anti-β-Amyloid, 1-16 Recombinant (clone 6E10.Rec) on formalin-fixed paraffin-embedded human normal brain (panel A) or human Alzheimer's brain (panel B). Following antigen retrieval using 88% formic acid, the tissue was incubated without (-) or with (+) 5.0 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB, Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 6E10Rec_PURE_beta-amyloid_Antibody_2_052423.png
    Beta-amyloid 1-40 peptide (red) or beta-amyloid 17-40 peptide (blue) were coated onto 96-well ELISA plates at 1 µg/mL and were then incubated with a dilution series of purified anti-β-Amyloid, 1-16 Recombinant (clone 6E10.Rec) for 2 hours at room temperature. Bound antibody was detected with HRP Goat anti-mouse IgG (Cat. No. 405306) and TMB substrate set (Cat. No. 421101). Absorbance was measured at 450 nm.
  • 6E10Rec_PURE_beta-amyloid_Antibody_3_052423.png
    Whole cell extracts (15 µg total protein) from human normal brain and human Alzheimer’s brain were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL purified anti-β-Amyloid, 1-16 Recombinant (clone 6E10.Rec) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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618251 25 µg £127
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618252 100 µg £315
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Description

Alzheimer's disease is characterized by the accumulation of aggregated β-amyloid (Aβ) peptides in senile plaques and vascular deposits. Aβ peptides are derived from amyloid precursor proteins (APP) through sequential proteolytic cleavage of APP by β-secretases and γ-secretases generating diverse Aβ species. Aβ can aggregate to form soluble oligomeric species and insoluble fibrillar or amorphous assemblies. Some forms of the aggregated peptides are toxic to neurons.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
β-Amyloid signal peptide residues 1-16
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Direct ELISA, WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/mL is suggested. Direct ELISA detects a β-amyloid fragment containing amino acids 1-16. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3068053 (BioLegend Cat. No. 618251)
AB_3068053 (BioLegend Cat. No. 618252)

Antigen Details

Structure
Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.
Distribution

Tissue distribution: Primarily nervous system, but also adipose tissue, intestine, and muscle
Cellular distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and Golgi apparatus

Function
The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes, protection against oxidative stress, regulation of cholesterol transport, transcription factor, and as an anti-microbial agent.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Antigen References
  1. Kumar A, et al. 2015. Pharmacol Rep. 67:195-203.
  2. Sadigh-Eteghad S, et al. 2015. Med Princ Pract. 24:1-10.
  3. Hampel H, et al. 2015. Expert Rev Neurother. 15:83-105.
  4. Puig KL & Combs CK. 2012. Exp Gerontol. 48: 608-11.
  5. Selkoe DJ & Hardy J. 2016. EMBO Mol Med. 8:595-608.
  6. Walsh DM & Selkoe DJ. 2007. J Neurochem. 101:1172-84.
Gene ID
351 View all products for this Gene ID
UniProt
View information about beta-Amyloid 1-16 on UniProt.org

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Go To Top Version: 1    Revision Date: 05/24/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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