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Purified anti-β-Amyloid, x-42 Recombinant Antibody

Pricing & Availability
Clone
BA3-9.R (See other available formats)
Regulatory Status
RUO
Other Names
AAA, ABETA, ABPP, AD1, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Isotype
Rabbit IgG
Ave. Rating
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Product Citations
publications
BA3-9dotR_PURE_beta-amyloid_Antibody_1and2_031918
IHC staining of anti-β-amyloid, x-42 antibody (clone BA3-9.R) on formalin-fixed paraffin-embedded normal (left panel) and Alzheimer's disease (right panel) brain tissue. Following antigen retrieval using 70% formic acid, the tissues were incubated with 1µg/mL of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Cat. No. 929901) was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm
  • BA3-9dotR_PURE_beta-amyloid_Antibody_1and2_031918
    IHC staining of anti-β-amyloid, x-42 antibody (clone BA3-9.R) on formalin-fixed paraffin-embedded normal (left panel) and Alzheimer's disease (right panel) brain tissue. Following antigen retrieval using 70% formic acid, the tissues were incubated with 1µg/mL of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Cat. No. 929901) was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm
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851601 25 µg £89
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851602 100 µg £221
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Description

Alzheimer's disease is characterized by the accumulation of aggregated Aβ peptides in senile plaques and vascular deposits. Aβ peptides are derived from amyloid precursor protein (APP) through sequential proteolytic cleavage of APP by β-secretases and γ-secretases generating diverse Aβ species. Aβ can aggregate to form soluble oligomeric species and insoluble fibrillar or amorphous assemblies. Some forms of the aggregated peptides are toxic to neurons.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Rabbit
Immunogen
C-terminus of A Beta 1-42 peptide
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This recombinant antibody clone is derived from clone BA3-9.

RRID
AB_2728579 (BioLegend Cat. No. 851601)
AB_2728579 (BioLegend Cat. No. 851602)

Antigen Details

Structure
Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.
Distribution

Tissue distribution: Primarily nervous system, but also adipose tissue, intestine, muscle.
Cellular distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.

Function
The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Antigen References
  1. Kumar A, et al. 2015. Pharmacol Rep. 67(2):195.
  2. Sadigh-Eteghad S, et al. 2015. Med Princ Pract. 24(1):1.
  3. Hampel H, et al. 2015. Expert Rev Neurother. 15(1):83.
  4. Puig KL, Combs CK. 2012. Exp Gerontol. 48(7): 608.
  5. Selkoe DJ, Hardy J. 2016. EMBO Mol Med. 8(6):595.
  6. Walsh DM, Selkoe DJ. 2007. J. Neurochem. 101(5):1172.
Gene ID
351 View all products for this Gene ID
UniProt
View information about beta-Amyloid x-42 on UniProt.org
Go To Top Version: 2    Revision Date: 03/26/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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