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Purified anti-β-Tubulin Antibody (Previously Covance catalog# MMS-410P)

Pricing & Availability
Clone
TU27/Tubulin (See other available formats)
Regulatory Status
RUO
Other Names
Tubulin, beta 2A
Previously
Covance Catalog# MMS-410P
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_TU27slashTubulin_PURE_beta-Tubulin_Antibody_WB_112817
Western blot analysis of cell lysates from HeLa (Human), Raw264.7 (Mouse), UMR106 (Rat), CHO (Hamster) and COS-7 (Monkey) using β-Tubulin Mouse primary antibody (clone TU27/Tubulin, 1:1000 dilution) and HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control (1:8000 dilution).
  • 1_TU27slashTubulin_PURE_beta-Tubulin_Antibody_WB_112817
    Western blot analysis of cell lysates from HeLa (Human), Raw264.7 (Mouse), UMR106 (Rat), CHO (Hamster) and COS-7 (Monkey) using β-Tubulin Mouse primary antibody (clone TU27/Tubulin, 1:1000 dilution) and HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control (1:8000 dilution).
  • 4_TU27-Tubulin_PURE_beta-Tubulin_Antibody_010620
    Western blot of purified anti-β-Tubulin antibody (clone TU27/Tubulin). Lane 1: Molecular weight marker; Lane 2: 30 µg of Drosophila head lysate; Lane 3: 30 µg of Drosophila S2 (embryonic) cell lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • TU27_Purified_beta_Tubulin_Antibody_WB_032715
    Western blot probed with MMS-410P anti-Tubulin antibody (TU 27) Lane1 : Molecular weight ladder; Lane2: Rat Brain Extract; Lane 3: Rat Brain Extract Lane 4: HeLa cell lysate
  • TU27_Purified_b-tubulin_Antibody_IHC_021315
    HeLa cells stained with polyclonal Giantin (green) used at 1:500 and monoclonal TU27 (red) used at 1:2000.
Cat # Size Price Quantity Check Availability Save
903401 250 µL £268
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Description

Tubulin is ubiquitously expressed and is the major constituent of microtubules, which are composed of alpha and beta tubulin heterodimers. There are five distinct forms of tubulin: alpha, beta, gamma, delta, and epsilon. Beta tubulin has multiple isoforms that are expressed in mammalian tissues and localize to different places in the cell.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, Non-Human Primate, Hamster, Drosophila
Reported Reactivity
Other species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was generated against taxol-assembled microtubules.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB, ICC, IHC
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition.

• WB: 1 µg/mL (1:1000)
• IF: 1,000

Application Notes

Reactivity to Drosophila was only verified with the purified format.

This antibody is effective in immunoblotting and immunostaining.

*Predicted MW = 55 kD

The TU27 clone is broadly reactive to b-tubulin subtypes and binds outside of the C-terminal isotype domains, but does not cross react with alpha-tubulin by immunoblotting. It recognizes a highly conserved epitope and reacts with ß-tubulin found in vertebrate as well as invertebrate tissues.

Additional Product Notes

For Western blotting, the suggested use of this reagent is 1.0 - 10 µg/mL in Drosophila and 1.0 µg/mL in other species.

Application References

(PubMed link indicates BioLegend citation)
  1. MK Lee, et al. 1990. Cell Motil. Cytoskel. 17:118.
  2. JE Alexander, et al. 1991. Proc Natl Acad Sci USA. 88:4685.
  3. J Lisztwan, et al. 1998. EMBO J. 17:368.
  4. Fujita T, et al. 2015. PLoS One. 10:e0116579. (WB) PubMed
  5. Ai M, et al. 2013. J. Neurosci. 33:10741. (WB) PubMed
Product Citations
  1. Venkatesan A, et al. 2019. Int J Mol Sci. 20:4. PubMed
  2. Mitra S, et al. 2021. Dev Biol. 478:205. PubMed
  3. Lence T, et al. 2016. Nature. 540:242-247. PubMed
  4. Courgeon M, et al. 2018. J Cell Sci. 131:jcs220251. PubMed
  5. Heine D, et al. 2016. Mycologia. 108: 581 - 589. PubMed
  6. Banerjee SJ, et al. 2021. PLoS One. 16:e0256738. PubMed
  7. Ho CH, et al. 2022. EMBO Rep. 23:e53231. PubMed
  8. Ai M, et al. 2013. J Neurosci. 33:10741-10749. PubMed
  9. Fujita T, et al. 2015. PLoS One. 10:e0116579. PubMed
  10. Astigarraga S, et al. 2018. Development. 145:. PubMed
  11. de Jesus AA, et al. 2019. J Allergy Clin Immunol. 143:1939. PubMed
  12. Swiatlowska P, et al. 2020. Nanoscale. 12:16315. PubMed
  13. Sharabi A, et al. 2019. JCI Insight. 4:e126294. PubMed
  14. Sah M, et al. 2020. Neurobiology of Disease. 78.45694444. PubMed
  15. Bawankar P, et al. 2021. Nat Commun. 12:3778. PubMed
  16. Vernia S et al. 2016. eLife. 5 pii: e17672. PubMed
  17. Knuckles P, et al. 2018. Genes Dev. 29:13. PubMed
  18. Church TW, et al. 2021. Elife. 10:. PubMed
  19. Kwon OS, et al. 2021. Nat Commun. 12:1351. PubMed
  20. Wan J, et al. 2016. Brain. 139: 2877 - 2890. PubMed
RRID
AB_2565030 (BioLegend Cat. No. 903401)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Microtubules
Gene ID
533307 View all products for this Gene ID
UniProt
View information about beta-Tubulin on UniProt.org
Go To Top Version: 7    Revision Date: 09/22/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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