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Purified anti-BIRC3 (C-IAP2) Antibody

Pricing & Availability
Clone
W17221A (See other available formats)
Regulatory Status
RUO
Other Names
Baculoviral IAP Repeat Containing 3, Inhibitor Of Apoptosis Protein 1, Apoptosis Inhibitor 2, TNFR2-TRAF-Signaling Complex Protein 1, RNF49, MIHC, API2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W17221A_PURE_BIRC3_Antibody_1_070519
Whole cell extracts (15 µg protein) from MCF7 (negative control), HaCaT and A431 (reduced expression controls), Daudi, Ramos, and A20 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-BIRC3 (c-IAP2) Antibody, clone W17221A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • W17221A_PURE_BIRC3_Antibody_1_070519
    Whole cell extracts (15 µg protein) from MCF7 (negative control), HaCaT and A431 (reduced expression controls), Daudi, Ramos, and A20 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-BIRC3 (c-IAP2) Antibody, clone W17221A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
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667701 25 µg £70
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667702 100 µg £174
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Description

Baculoviral IAP Repeat Containing Protein 3, or BIRC3, is a member of the Inhibitor of apoptosis (IAP) family of proteins initially characterized as a component of the TNFα receptor signaling complex. BIRC3 functions as an E3 ubiquitin ligase, polyubiqiutinating target proteins such as RIP kinases, caspases, TRAF1, and BCL10. Loss of BIRC3 results in a significant impairment of TNFα-mediated NF-κB activation signaling cascades.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant protein corresponding to amino acids 355-604 of human BIRC
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone has not been tested for IP.

This clone failed to test 4% PFA-fixed Ramos cells permabilized with either methanol or Triton X-100; we do not recommend using the clone for ICC.

This clone produced additional bands between ~65 and 95 kD when tested by WB. These bands were also observed with a control antibody raised with a separate epitope. These bands may represent ubiquitinated BIRC3.

Product Citations
  1. Soh KT, et al. 2016. Curr Protoc Cytom. 7.45.1:75. PubMed
RRID
AB_2814506 (BioLegend Cat. No. 667701)
AB_2814506 (BioLegend Cat. No. 667702)

Antigen Details

Structure
BIRC3 is a 604 amino acid protein with a predicted molecular weight of 68 kD.
Distribution

Nucleus, cytoplasm/widely expressed

Function
Inhibits apoptosis
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Innate Immunity, Signal Transduction, Ubiquitin/Protein Degradation
Antigen References
  1. Seitz CS, et al. 2000. J. Clin. Invest. 105:253.
  2. Contze DB, et al. 2005. Mol Cel Biol. 25:3384.
  3. Rahal R, et al. 2014. Nat. Med. 20:87.
Gene ID
330 View all products for this Gene ID
UniProt
View information about BIRC3 on UniProt.org
Go To Top Version: 1    Revision Date: 07/05/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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