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Purified anti-Blimp-1 Antibody

Pricing & Availability
Clone
ROS195G (See other available formats)
Regulatory Status
RUO
Other Names
B lymphocyte-induced maturation protein-1, PRDM-1, BLIMP1
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
ROS195G_PURE_Blimp-1_Antibody_0_WB_090916
Total cell lysates (15 µg total protein) from HEPG2 (negative control), A549 (negative control), SR and RPMI8226 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 2.0 µg/mL of Purified anti-Blimp1 Antibody, clone ROS195G, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • ROS195G_PURE_Blimp-1_Antibody_0_WB_090916
    Total cell lysates (15 µg total protein) from HEPG2 (negative control), A549 (negative control), SR and RPMI8226 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 2.0 µg/mL of Purified anti-Blimp1 Antibody, clone ROS195G, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • ROS195G_PURE_Blimp-1_Antibody_1_WB_090916
    Total cell lysates (15 µg protein) from U266, Jurkat and Raji were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with anti-Blimp-1 (clone ROS195G). Proteins were visualized using a goat anti-mouse IgG secondary antibody conjugated to HRP and chemiluminescence detection. Purified anti-GAPDH antibody was used as a loading control.
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648202 100 µg £182
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Description

Blimp-1 is a 98 Kd protein containing five Kuppel-type zinc finger domains that confer sequence specific DNA binding.  It has been postulated to be a master regulator of terminal B cell differentiation. In the BCL-1 lymphoma model of differentiation from mature B cells to plasma cells, ectopic expression of Blimp-1 is sufficient to cause terminal differentiation manifested by loss of surface immunoglobulin, IgM secretion, expression of syndecan-1 and cessation of cell division. Recently, it is reported that Blimp-1 is also involved in T cell differentiation and T cell homeostasis and suppression of IL-2 production.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant GST-blimp-1 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC, IP, ICC, ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Garcia JF, et al. 2006. Haematogica 91:467.
  2. Travert M, et al. 2012. Blood. 119:5795. PubMed
  3. Tsao HW, et al. 2013. PNAS. 110:15776. PubMed
Product Citations
  1. Tsao H, et al. 2013. Proc Natl Acad Sci U S A. 110:15776. PubMed
  2. Yu L, et al. 2020. Cell Stem Cell. 28(3):550-567.e12. PubMed
  3. Travert M, et al. 2012. Blood. 119:5795. PubMed
  4. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
RRID
AB_2300132 (BioLegend Cat. No. 648202)

Antigen Details

Structure
98 Kd protein containing five Kruppel-type zinc finger domains
Distribution

Expressed in CD4+ and CD8+ effector/memory T cells and in Foxp3+ T reg cells. In the B cell lineage, it is specifically expressed in the antibody secreting cells.

Function
A transcription repressor expressed in B and T cells.
Cell Type
B cells, T cells, Tregs
Biology Area
Immunology
Molecular Family
Nuclear Markers
Antigen References

1. Zhao WL, et al. 2008. Blood 111:3867.
2. Climmino L, et al. 2008. J. Immunol. 181:2338.
3. Martins G and Calame K. 2008. Annu Rev Immunol. 26:133.

Gene ID
639 View all products for this Gene ID
UniProt
View information about Blimp-1 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All Blimp-1 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-Blimp-1 ROS195G WB,IHC,IP,ICC,ELISA
Go To Top Version: 4    Revision Date: 08/17/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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