Purified anti-BRST-1 Antibody

Pricing & Availability
Clone
CU-18 (See other available formats)
Regulatory Status
RUO
Other Names
EPCAM, Epithelial cell adhesion molecule, epithelial glycoprotein 314, Tumor-Associated Calcium Signal Transducer 1, Adenocarcinoma-Associated Antigen, Cell Surface Glycoprotein Trop-1, CD326 Antigen
Isotype
Mouse IgG1, κ
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Product Citations
publications
CU-18_PURE_BRST-1_Antibody_IHC-P_061016
IHC staining of anti-BRST-1 antibody (clone CU-18) on formalin-fixed paraffin-embedded human breast tumor tissue. The tissue was incubated with the primary antibody at 1.47 µg/ml for 20 minutes at room temperature. BioLegend's Ultra Streptavidin (USA) HRP Kit (Multi-Species) was used for detection followed by counterstaining with hematoxylin.
  • CU-18_PURE_BRST-1_Antibody_IHC-P_061016
    IHC staining of anti-BRST-1 antibody (clone CU-18) on formalin-fixed paraffin-embedded human breast tumor tissue. The tissue was incubated with the primary antibody at 1.47 µg/ml for 20 minutes at room temperature. BioLegend's Ultra Streptavidin (USA) HRP Kit (Multi-Species) was used for detection followed by counterstaining with hematoxylin.
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915004 100 µg £157
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Description

BRST1 is a carcinoma-associated antigen and a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was generated against a protein secreted by the T47D (clone 11) human breast carcinoma cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

CU-18 detects a human breast carcinoma associated glycoprotein BCA-225, a 225 kD glycoprotein secreted by the T47D breast carcinoma cell line.

Application References

(PubMed link indicates BioLegend citation)
  1. Imam SA, et al. 1995. Anticancer Res. 15:1153. PubMed
  2. Ansai S, et al. 1994. J. Dermatol. 21(1):20. PubMed
  3. Ma CK, et al. 1993. Am. J. Clin. Pathol. 99(5):551. PubMed
RRID
AB_2616963 (BioLegend Cat. No. 915004)

Antigen Details

Structure
BRST-1 is a 35 kD protein.
Distribution

Cytoplasm, extracellular, and nucleus.

Function
BRST-1 may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. BRST-1 plays a role in embryonic stem cells proliferation and differentiation.
Biology Area
Cell Adhesion, Cell Biology
Gene ID
4072 View all products for this Gene ID
UniProt
View information about BRST-1 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06/10/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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