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Purified anti-BRST-3 Antibody

Pricing & Availability
Clone
B72.3 (See other available formats)
Regulatory Status
RUO
Other Names
BRST3
Isotype
Mouse IgG1, κ
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Product Citations
publications
B72point3_PURE_BRST3_Antibody_1_081718
IHC staining of purified anti-BRST-3 antibody (clone B72.3) on formalin-fixed paraffin-embedded human breast tumor tissue. The tissue was incubated with 4 µg/ml of the primary antibody for 20 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • B72point3_PURE_BRST3_Antibody_1_081718
    IHC staining of purified anti-BRST-3 antibody (clone B72.3) on formalin-fixed paraffin-embedded human breast tumor tissue. The tissue was incubated with 4 µg/ml of the primary antibody for 20 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • B72point3_PURE_BRST3_Antibody_2_081718
    IHC staining of purified anti-BRST-3 antibody (clone B72.3) on formalin-fixed paraffin-embedded human breast tumor tissue. The tissue was incubated with 4 µg/ml of the primary antibody for 20 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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915206 100 µg £157
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Description

BRST-3, also known as TAG-72 (Tumor Associated Glycoprotein-72), a high molecular weight (220-400 kD) glycoprotein complex, is expressed by a wide variety of human adenocarcinomas.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was generated against a membrane-enriched fraction of a breast carcinoma metastatic to the liver.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 2.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

B72.3 is reactive with a broad spectrum of epithelial malignancies in human, including invasive ductal carcinoma, lung carcinomas, ovarian, gastric and colonic adenocarcinomas. It appears to be non-reactive or weakly reactive to most normal tissues.

Application References

(PubMed link indicates BioLegend citation)
  1. Liu G, et al. 2008. Eur. J. Nucl. Med. Mol. Imaging 35(2):272.
  2. Lyda MH, et al. 2000. Hum. Pathol. 31(8):980.
  3. Clemo FA, et al. 1995. Vet. Pathol. 32:147. (IHC)
  4. Colcher D, et al. 1981. Proc. Natl. Acad. Sci. USA 78:3199.
RRID
AB_2616965 (BioLegend Cat. No. 915206)

Antigen Details

Biology Area
Cancer Biomarkers, Cell Biology
Gene ID
NA
UniProt
View information about BRST-3 on UniProt.org
Go To Top Version: 3    Revision Date: 08/17/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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