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Purified anti-C/EBP β (2 isoforms C/EBP β, LAP) Antibody

Pricing & Availability
Clone
A16 (See other available formats)
Regulatory Status
RUO
Other Names
CCAAT/enhancer-binding protein beta, Alpha-1-acid glycoprotein/enhancer binding protein, Interleukin 6-dependent DNA-binding protein (IL-6DBP), Nuclear factor of interleukin 6 (NF-IL6), Liver-enriched transcriptional activator protein (LAP)
Isotype
Mouse IgG1, κ
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Product Citations
publications
A16_PURE_CslashEBP_Antibody_WB_062618
Total lysates (15 µg protein) from P388D1 (lane 1) and NIH3T3 (lane 2) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1 µg/mL Purified anti-C/EBP β antibody (1:500 dilution), clone A16 (upper blot). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-C/EBP β antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin antibody, clone 2F1-1 (lower). Lane M: Molecular weight ladder.
  • A16_PURE_CslashEBP_Antibody_WB_062618
    Total lysates (15 µg protein) from P388D1 (lane 1) and NIH3T3 (lane 2) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1 µg/mL Purified anti-C/EBP β antibody (1:500 dilution), clone A16 (upper blot). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-C/EBP β antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin antibody, clone 2F1-1 (lower). Lane M: Molecular weight ladder.
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626001 25 µg £70
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626002 100 µg £150
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Description

C/EBPβ (also known as CCAAT/enhancer-binding protein beta, interleukin 6-dependent DNA-binding protein, and nuclear factor of interleukin 6 [NF-IL6]) is a member of the basic leucine zipper C/EBP transcription factor family. Mouse C/EBP has three isoforms that include C/EBPβ-FL, C/EBPβ-LAP, and C/EBPβ-LIP (31kD, 29kD and 16kD, respectively). This protein is localized in the nucleus and functions as a transcription factor in immune, inflammatory, acute-phase, cytokine, collagen type I gene expression. It is upregulated by growth hormones and has been shown to interact with CEBP-α, CEBP-δ, CEBP-γ, CEBP-ε, and CEBP-ζ. The C/EBPβ protein is known to be modified by phosphorylation and ubiquitination. Clone A16 has been shown to be useful for Western blotting to recognize C/EBPβ-FL (31kD) and C/EBPβ-LAP (29kD) isoforms.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant full length mouse C/EBP β protein
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

A16 monoclonal antibody recognizes C/EBPß-FL (31kD) and C/EBPß-LAP (29kD) isoforms, but not C/EBPß-LIP (16kD).

Application References

(PubMed link indicates BioLegend citation)
  1. Lee YM, et al.1996. Mol. Cell. Biol. 16:4257
  2. Su, et al. 2003. J. Biol. Chem. 278:51150.
Product Citations
  1. Adayev T, et al. 2014. BMC Med Genet. 119:15. PubMed
  2. Brina D, et al. 2015. Nat Commun. 6: 8261. PubMed
RRID
AB_2097741 (BioLegend Cat. No. 626001)
AB_2097741 (BioLegend Cat. No. 626002)

Antigen Details

Structure
C/EBP transcription factor family, basic leucine zipper, dimer. Mouse C/EBP has three isoforms that include C/EBPβ-FL, C/EBPβ-LAP, and C/EBPβ-LIP (31kD, 29kD and 16kD, respectively).
Distribution

Nuclear

Function
Transcription factor in immune, inflammatory, acute-phase, cytokine, collagen type I genes
Interaction
CEBP-α, CEBP-β, CEBP-δ, CEBP-γ, CEBP-ε, CEBP-ζ
Modification
Phosphorylation, Ubiquitination
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Chang C, et al. 1990. Mol. Cell Biol. 10:6642.
2. Eaton E, et al. 2001. J. Cell Physiol. 189:91.
3. Piwien-Pilipuk G, et al. 2002. J. Biol. Chem. 277:44557.
4. Hattori T, et al. 2003. Oncogene. 22:1273.

Regulation
Growth hormone
Gene ID
12608 View all products for this Gene ID
UniProt
View information about C/EBP beta on UniProt.org
Go To Top Version: 3    Revision Date: 12/17/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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