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Purified anti-C/EBPα Antibody

Pricing & Availability
Clone
16C12B70 (See other available formats)
Regulatory Status
RUO
Other Names
CCAAT/enhancer-binding protein alpha, C/EBP-A
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
16C12B70_CEBPa_purified_Antibody_1_WB_031919
Total cell lysates (15 µg total protein) from Jurkat (negative control), THP-1, and RAW264.7 cells were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL of Purified anti-C/EBPα Antibody, clone 16C12B70, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 664804) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker.
  • 16C12B70_CEBPa_purified_Antibody_1_WB_031919
    Total cell lysates (15 µg total protein) from Jurkat (negative control), THP-1, and RAW264.7 cells were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL of Purified anti-C/EBPα Antibody, clone 16C12B70, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 664804) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker.
  • 16C12B70_CEBPa_purified_Antibody_2_ICC_011321
    THP-1 cells were stained with purified anti-C/EBPα (clone 16C12B70) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG (green) antibody and Alexa Fluor® 594 conjugated phalloidin (red).
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662102 100 µg £182
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Description

The CCAAT-enhancer binding proteins (C/EBP) are members of the basic region/leucine zipper (bZIP) transcription factors, that bind to the consensus CCAAT sequence in DNA. C/EBPα is expressed in two isoforms: p42 and p30 by ultilizing different translational start codons. The C/EBPα p30 isoform has little to no transactivating activity and acts as an inhibitor. C/EBPα regulates expression of various myeloid-specific genes and is required for myeloid differentiation. C/EBPα has been found to play a key role in adipogenesis, since inhibition of C/EBPα blocks adipocyte differentiation. C/EBPα also regulates cell cycle progression through interactions with cyclin-dependent kinases (CDK) and by inducing the expression of CDK inhibitor p21. Mutations in the C/EBPα gene are associated with the development of acute myeloid leukemia.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide (AKQLALAGLFPYQPPPP) conjugated to KLH.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product weakly reacts with Mouse.

Product Citations
  1. Oliveros-Matus P, et al. 2020. Front Pharmacol. 11:303. PubMed
  2. Li Y, et al. 2018. Front Immunol. 32:415. PubMed
RRID
AB_2564224 (BioLegend Cat. No. 662102)

Antigen Details

Structure
358 amino acids with a predicted molecular weight of 38 kD; contains a bZIP domain responsible for DNA binding.
Distribution

Nucleus

Function
C/EBPα is a bZIP transcription factor that regulates proliferation and differentiation of myeloid cells and adipocytes.
Interaction
C/EBPα forms heterodimers with C/EBPβ and γ; interacts with CDK2, CDK4, PRDM16, UBN1, and ZNF638.
Cell Type
Neutrophils
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. De Obaldia ME, et al. 2013. Nat. Immunol. 14:1277.
2. Di Tullio A, et al. 2011. Proc. Natl. Acad. Sci. USA. 108:17016.
3. Fan HY, et al. 2011. Mol. Endocrinol. 25:253.
4. Cai DH, et al. 2008. Oncogene. 27:2772.
5. Khanna-Gupta A. 2008. Blood Cells Mol. Dis. 41:77.
6. Janardhan HP. 2008. Sci. Signal. 1:jc2.

Gene ID
1050 View all products for this Gene ID
UniProt
View information about C/EBPalpha on UniProt.org
Go To Top Version: 5    Revision Date: 08/11/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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