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Purified anti-C2H2 zinc finger protein phospho linker region (HpTGEKP) Antibody

Pricing & Availability
Clone
18E9.D9 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A-18E9-D9_PURE_C2H2_zincfinger_Antibody_1_071519
Whole cell extracts (15 µg protein) from serum starved HeLa untreated (-) or treated (+) with 200 ng/mL Nocodazole for 20 hrs were resolved on a 4-12% Bis-Tris gel and transferred to a PVDF membrane. The membrane was treated (+ LPP) or untreated (- LPP) with lambda protein phosphatase, overnight at 4°C and probed with 1.0 µg/mL (1:500 dilution) of purified anti-C2H2 zinc finger protein phospho linker region (HpTGEKP), clone 18E9.D9, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • A-18E9-D9_PURE_C2H2_zincfinger_Antibody_1_071519
    Whole cell extracts (15 µg protein) from serum starved HeLa untreated (-) or treated (+) with 200 ng/mL Nocodazole for 20 hrs were resolved on a 4-12% Bis-Tris gel and transferred to a PVDF membrane. The membrane was treated (+ LPP) or untreated (- LPP) with lambda protein phosphatase, overnight at 4°C and probed with 1.0 µg/mL (1:500 dilution) of purified anti-C2H2 zinc finger protein phospho linker region (HpTGEKP), clone 18E9.D9, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • B-18E9pointD9_PURE_C2H2_zincfinger_Antibody_041216
    Total cell lysate (15 µg protein) from synchronized HeLa and NIH3T3 cells and from M-phase synchronized HeLa and NIH3T3 cells (300 nM nocodazole treatment for 24 hours) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-C2H2 zinc finger protein phospho linker region (HpTGEKP) (clone 18E9.D9) antibody. Proteins were visualized using an HRP Goat anti-mouse-IgG antibody and chemiluminescence detection.
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685702 100 µg £230
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Description

C2H2 zinc finger proteins (ZFPs) are DNA-binding transcription factors that regulate a variety of biological processes such as cellular growth, differentiation, and proliferation. A zinc finger protein can contain several tandems of clustered zinc finger motifs responsible for DNA binding. However, stable ZFP binding to DNA requires the conserved linker sequences that join adjacent zinc fingers. These linkers participate in wrapping of the ZFP around the double helix of DNA, which is required for efficient binding. Most of the ZFP linkers contain the same amino acid sequence HTGEKP, which has been shown to be phosphorylated at the threonine residue by TOPK/PBK during mitosis. Phosphorylation on the linker sequence of ZFP leads to the inactivation of DNA binding activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The antibody recognizes the mitotically-phosphorylated form of the HTGEKP conserved motif of C2H2 Zinc finger proteins. This is conserved across most higher eukaryotic species and is found in mitotic cells of human and mouse (and most eukaryotic) species.

RRID
AB_2616798 (BioLegend Cat. No. 685702)

Antigen Details

Structure
Conserved linker region (HTGEKP) of C2H2 zinc finger proteins.
Distribution

Mitotic cells.

Function
The linker region (HTGEKP) of C2H2 zinc finger proteins facilitate stable DNA binding and is highly conserved among these zinc finger proteins. During mitosis, C2H2 zinc finger proteins are phosphorylated on the threonine residue of the linker peptide, which result in the inactivation of their DNA binding activity.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Transcription Factors
Molecular Family
Phospho-Proteins
Antigen References

1. Dovat S, et al. 2002. Genes Dev. 16:2985.
2. Rizkallah R, et al. 2011. Cell Cycle 10:3327.
3. Rizkallah R, et al. 2015. Oncotarget. 6:1446.

Gene ID
NA
UniProt
View information about C2H2 zinc finger proteins on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 07/15/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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