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Purified anti-CA125 Antibody

Pricing & Availability
Clone
618F (See other available formats)
Regulatory Status
RUO
Other Names
Mucin-16, MUC-16, MUC16, Ovarian cancer-related tumor marker CA125, CA-125, Ovarian carcinoma antigen CA125, CA125
Isotype
Mouse IgG1
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Product Citations
publications
618F_PURE_Liquid_CA125_Antibody_1_WB_Antibody_031919
Total cell lysates (15 µg total protein) from HEK293 and Daudi (negative controls), HaCat and A431 (positive controls cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL of Purified anti-CA125 Antibody, clone 618F, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • 618F_PURE_Liquid_CA125_Antibody_1_WB_Antibody_031919
    Total cell lysates (15 µg total protein) from HEK293 and Daudi (negative controls), HaCat and A431 (positive controls cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL of Purified anti-CA125 Antibody, clone 618F, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • 618F_PURE_Liquid_CA125_Antibody_2_IHC_Antibody_072816
    IHC staining of anti-CA125 (clone 618F) antibody on formalin-fixed paraffin-embedded human ovarian tumor. Following antigen retrieval using Retrieve-All Antigen Unmasking System's Retrieve-All 1 (Universal pH 8.0), the tissue was incubated with the primary antibody at 0.5 µg/ml for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit was used for detection, followed by counterstaining with hematoxylin.
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666904 100 µg £221
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Description

CA125 is believed to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. It binds to MSLN, which mediates heterotypic cell adhesion. This may contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium through its binding to MSLN.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Endogenous protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
FC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. For immunohistochemistry, a concentration range of 0.5 - 5 µg per ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The observed molecular weight of this target is ~500 kD, which is considerably different than the predicted MW of 1519 kD. Similar observed molecular weights were confirmed using control antibodies from other vendors and have been reported by other vendors.

Application References

(PubMed link indicates BioLegend citation)
  1. Goodell CA, et al. 2009. J. Ovarian Res. 2:8. (FC)
RRID
AB_2629540 (BioLegend Cat. No. 666904)

Antigen Details

Structure
CA125 is a 250 kD+ protein.
Biology Area
Cancer Biomarkers, Cell Biology
Antigen References

1. Yin BW, et al. 2001. J. Biol. Chem. 276:27371.
2. Duraisamy S, et al. 2006. Gene. 373:28.
3. Bast RC Jr, et al. 1998. Int. J. Biol. Markers 13:179.

Gene ID
94025 View all products for this Gene ID 73732 View all products for this Gene ID
UniProt
View information about CA125 on UniProt.org
Go To Top Version: 2    Revision Date: 10/07/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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