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Purified anti-Caldesmon Antibody

Pricing & Availability
Clone
W22071A (See other available formats)
Regulatory Status
RUO
Other Names
Caldesmon 1, Caldesmon, CDM, H-CAD, L-CAD, H-CD, Testis Secretory Sperm-Binding Protein Li 227n, NAG22, LCAD, HCAD, CAD
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A. W22071A_PURE_Caldesmon_WB_121423
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 µg/mL Purified anti-Caldesmon (clone W22071A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Lane M: Molecular weight marker
  • A. W22071A_PURE_Caldesmon_WB_121423
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 µg/mL Purified anti-Caldesmon (clone W22071A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Lane M: Molecular weight marker
  • B. W22071A_PURE_CALD1_ICC_102423
    U-87 MG cells were fixed with Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with 5 µg/mL of Purified anti-Caldesmon (clone W22071A), followed by with Alexa Fluor® 594 Goat anti-rat IgG (Cat. No. 405422) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured by a Revvity Operetta CLS™ High Content Analysis System with a 60X objective. Scale bar: 50 µm
  • C. W22071A_PURE_CALD1_IHC-P_102423
    IHC staining of Purified anti-Caldesmon (clone W22071A) on formalin-fixed paraffin-embedded human uterus (positive tissue, panel A and C) and skeletal muscle (negative tissue, panel B and D). Following antigen retrieval using Citrate buffer, 10X (Cat. No. 420902), the tissue was incubated with 5 µg/mL of Purified anti-Caldesmon (clone W22071A) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 10X (panel A and B) or 40X (panel C and D) objective. Scale bar: 50 µm
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629851 25 µg £124
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629852 100 µg £315
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Description

Caldesmon is a calmodulin-, actin-, and tropomyosin-binding protein that plays an essential role in smooth muscle and non-muscle contraction. This protein is also involved in cellular mitosis and Schwann cell migration during peripheral nerve regeneration.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human Caldesmon
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For immunohistochemistry (IHC-P), either Citrate Buffer, 10X (Cat. No, 420902) or Tris-EDTA pH 9.0 is recommended for antigen retrieval.

For immunocytochemistry (ICC), fixing with Fixation Buffer (Cat. No. 420801) is recommended. Alternatively, 4% paraformaldehyde fixation followed by either permeabilization with 0.5% Triton X-100 or ice-cold methanol is recommended.

Clone W22071A cross-reacts with mouse in western blotting (WB).

RRID
AB_3083418 (BioLegend Cat. No. 629851)
AB_3083418 (BioLegend Cat. No. 629852)

Antigen Details

Structure
Caldesmon is a 793 amino acid protein with a predicted molecular mass of 93 kD
Distribution

Tissue distribution: high-molecular-weight Caldesmon (isoform 1) is predominantly expressed in smooth muscle; low-molecular-weight Caldesmon (isoforms 2, 3, 4 and 5) is widely distributed in non-muscle tissues and cells. Caldesmon is not expressed in skeletal muscle or heart.
Cellular distribution: cytoskeleton, cytosol, and plasma membrane.

Function
Regulates actomyosin interactions in smooth muscle and non-muscle cells which affects cell contractions; involved in cellular mitosis and in Schwann cell migration during peripheral nerve regeneration.
Interaction
Calmodulin, actin, and tropomyosin
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure
Molecular Family
Enzymes and Regulators
Antigen References
  1. Huber PA. 1997. Int J Biochem Cell Biol. 29:1047-51.
  2. Mayanagi T and Sobue K. 2011. Cell. Adh. Migr. 5:150-9.
  3. Wang CL. 2008. Adv Exp Med Biol. 644:250-72.
  4. Sobue K and Sellers JR. 1991. J Biol Chem. 266:12115-8.
Gene ID
800 View all products for this Gene ID
UniProt
View information about Caldesmon on UniProt.org
Go To Top Version: 1    Revision Date: 11/20/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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