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Purified anti-CARD9 Antibody

Pricing & Availability
Clone
13D9C60 (See other available formats)
Regulatory Status
RUO
Other Names
Caspase Recruitment Domain-Containing Protein 9, CANDF2, hCARD9
Isotype
Mouse IgG2a, κ
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Product Citations
publications
13D9C60_PURE_CARD9_Antibody_1_WB_072315
Total cell lysates from THP-1 cells (lane 1), HeLa cells (lane 2), and RAW264.7 cells (lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal CARD9 (clone 13D9C60) antibody. Proteins were visualized using a HRP-conjugated anti-mouse-IgG secondary and chemiluminescence detection.
  • 13D9C60_PURE_CARD9_Antibody_1_WB_072315
    Total cell lysates from THP-1 cells (lane 1), HeLa cells (lane 2), and RAW264.7 cells (lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal CARD9 (clone 13D9C60) antibody. Proteins were visualized using a HRP-conjugated anti-mouse-IgG secondary and chemiluminescence detection.
  • 13D9C60_PURE_CARD9_Antibody_2_IF_090916
    HeLa cells were fixed with 2% (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for five minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2 µg/mL anti-CARD9 Antibody (clone 13D9C60) followed by Alexa Fluor® 594 (red) conjugated goat anti-mouse IgG in blocking buffer for two hours at room temperature. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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679102 100 µg £213
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Description

Caspase recruitment domain-containing protein 9 (CARD9) is a member of the CARD protein family, which is known to play regulatory roles in cell apoptosis. CARD9 is an adaptor protein that mediates the signaling downstream of C-type lectin receptors (CLRs), such as Dectin-1, Dectin-2, and Mincle. CARD9 also participates in the signaling downstream of the Retinoic acid-inducible gene I receptor (RIG-1) family. After stimulation of the receptors, CARD9 is phosphorylated and forms a trimolecular complex with B cell leukemia-lymphoma 10 (Bcl10) and mucosa-associated lymphoid tissue 1 (Malt1), which activates the canonical NF-κB pathway and leads to the induction of proimflammatory cytokines, including IL-6, IL-1β, and tumor necrosis factors-alpha (TNFs). CARD9 has been suggested to play an important role in antifungal host defense, as the CARD9 deficient mice are more susceptible to pathogenic fungi infections.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CARD9 peptide (LRKMQKGWRQGEEDRENT) conjugated to KLH.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Zhu Q, et al. 2023. Front Cardiovasc Med. 10:1125717. PubMed
RRID
AB_2566076 (BioLegend Cat. No. 679102)

Antigen Details

Structure
536 amino acids with a predicted molecular weight of 62.2 kD. Contains a N-terminal CARD domain responsible for interacting with other CARD-containing proteins.
Distribution

Cytoplasm.

Function
CARD9 is a scaffold molecule that mediates the assembly of a signaling complex containing BCL10 and MALT1. CARD9 plays an important role in processes such as apoptosis, NF-κB activation, and host defense against pathogens.
Interaction
Interacts with BCL10, NOD2, RIPK2, and VHL.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity, Neuroscience, Signal Transduction
Antigen References

1. Jia XM, et al. 2014. J. Exp. Med. 211:2307.
2. Roth S, et al. 2014. Nat. Immunol. 15:538.
3. Yamamoto H, et al. 2014. Infect Immun. 4:1606.
4. Shenderov K, et al. 2013. J. Immunol. 190:5722.
5. Drummond RA, et al. 2011. Eur. J. Immunol. 41:276.
6. Goodridge HS, et al. 2009. J. Immunol. 182:1146.

Gene ID
64170 View all products for this Gene ID
UniProt
View information about CARD9 on UniProt.org
Go To Top Version: 3    Revision Date: 06/12/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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