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Purified anti-Caspase-3 Antibody

Pricing & Availability
Clone
W20054B (See other available formats)
Regulatory Status
RUO
Other Names
Caspase 3 preproprotein, Casp3, cpp32, Apopain, Yama, PARP cleavage protease, SREBP cleavage activity 1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W20054B_PURE_Caspase-3_Antibody_120121
Total cell lysates (15 µg total protein) from Jurkat (Lane 1, untreated control) and Jurkat treated with 1 µM of staurosporine (Lane 2) for 3 hours were resolved by 4-12 % Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 μg/mL of anti-Caspase-3 antibody overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at 1:3000 dilution. Direct-Blot™ HRP anti-β actin antibody (Cat. No. 643807) was used as a loading control at a 1:10,000 dilution (lower). Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection reagent. Lane M: Molecular weight ladder
  • W20054B_PURE_Caspase-3_Antibody_120121
    Total cell lysates (15 µg total protein) from Jurkat (Lane 1, untreated control) and Jurkat treated with 1 µM of staurosporine (Lane 2) for 3 hours were resolved by 4-12 % Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 μg/mL of anti-Caspase-3 antibody overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at 1:3000 dilution. Direct-Blot™ HRP anti-β actin antibody (Cat. No. 643807) was used as a loading control at a 1:10,000 dilution (lower). Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection reagent. Lane M: Molecular weight ladder
Cat # Size Price Quantity Check Availability Save
949801 25 µg £81
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949802 100 µg £201
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Description

Caspase 3 is a member of the peptidase family C14. This cytoplasmic caspase functions as a heterotetramer and has two isoforms (one acts as a dominant negative inhibitor). The pro-form of this caspase is approximately 32 kD. Caspase 3 is involved in the caspase activation cascade responsible for apoptosis execution. Caspase 3 is inhibited by c-IAP1, c-IAP2, XIAP, survivin, and Livin and activated by Caspase 6, -8, and -10, granzyme B, Apaf-1 by cleavage into large p17 and small p12 subunits. Caspase 3 has been shown to cleave PARP, huntingtin, androgen receptor, atrophin-1, DNA- PKcs, D4-GDI, hnRNPs C1/C2, U1snRNP, PKC delta, ATM, fodrin, gelsolin, ICAD, RIP, X-IAP, STAT1, topoisomerase I, vimentin, Rb, lamin B, mdm2, and β-catenin. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human Caspase-3 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody recognizes endogenous human Caspase-3 proteins in both total and spliced forms from Jurkat cell lysates treated with or without staurosporine. 

RRID
AB_2904464 (BioLegend Cat. No. 949801)
AB_2904464 (BioLegend Cat. No. 949802)

Antigen Details

Structure
Caspase 3 is a 277 amino acid protein with a molecular mass of 32 kD.
Distribution

Nucleus and cytosol

Function
Caspase 3 regulates programmed cell death (apoptosis)
Interaction
Active heterodimers between large p17 subunit and small p11 subunit of Casp7 and vice versa. Cleaves PARP, huntingtin, androgen receptor, atrophin-1, DNA- PKcs, D4-GDI, hnRNPs C1/C2, U1snRNP, PKC delta, ATM, fodrin, gelsolin, ICAD, RIP, X-IAP, STAT1, topo
Cell Type
B cells, Endothelial cells, Fibroblasts, Monocytes, T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Death, Neuroscience
Antigen References
  1. Goldberg YP, et al. 1996. Nat Genet. 13:442-9.
  2. Fernandes-Alnemri T, et al. 1996. Proc Natl Acad Sci U S A. 93:7464-9.
  3. Slee EA, et al. 2001. J Biol Chem. 276:7320-6.
  4. Woo M, et al. 2003. Nat Immunol. 4:1016-22.
Gene ID
836 View all products for this Gene ID
UniProt
View information about Caspase-3 on UniProt.org
Go To Top Version: 1    Revision Date: 12/01/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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