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Purified anti-Caspase-3 Antibody

Pricing & Availability
Clone
Poly6341 (See other available formats)
Regulatory Status
RUO
Other Names
Caspase 3 preproprotein, Casp3, cpp32, Apopain, Yama, PARP cleavage protease, SREBP cleavage activity 1
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly6341 WB
Western blot analysis of extracts from Hela cells, using anti-caspase-3, Poly6341.
  • Poly6341 WB
    Western blot analysis of extracts from Hela cells, using anti-caspase-3, Poly6341.
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634101 125 µL £77
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Description

Caspase 3 is a member of the peptidase family C14. This cytoplasmic caspase functions as a heterotetramer and has two isoforms (one acts as a dominant negative inhibitor). The pro-form of this caspase is approximately 32 kD. Caspase 3 is involved in the caspase activation cascade responsible for apoptosis execution. Caspase 3 is inhibited by c-IAP1, c-IAP2, XIAP, survivin, and Livin and activated by Caspase 6, -8 , and -10, granzyme B, Apaf-1 by cleavage into large p17 and small p12 subunits. Caspase 3 has been shown to cleave PARP, huntingtin, androgen receptor, atrophin-1, DNA- PKcs, D4-GDI, hnRNPs C1/C2, U1snRNP, PKC delta, ATM, fodrin, gelsolin, ICAD, RIP, X-IAP, STAT1, topoisomerase I, vimentin, Rb, lamin B, mdm2, and β-catenin. The poly6341 antibody has been shown to be useful for western blotting, immunoprecipitation and immunohistochemistry of human Human caspase-3 precursor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Peptide mapping to the amino terminal domain of human caspase-3 prescursor
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% gelatin.
Preparation
The antibody was purified by affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. DO NOT FREEZE.
Application

WB - Quality tested
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.  Western blotting suggested working dilution(s): Use 25µl per 5 ml antibody dilution buffer for each mini-gel.  It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes


This product may contain other non-IgG subtypes.

Additional Product Notes
The 125 µL size can be used for approximately 5 Western blots.
Product Citations
  1. Linetsky M, et al. 2020. Free Radic Biol Med. 152:280. PubMed
  2. Ramón-Luing LA, et al. 2022. Pathogens. 11:. PubMed
RRID
SCR_001134 (BioLegend Cat. No. 634101)

Antigen Details

Structure
Peptidase family C14, heterotetramer. Two isoforms, pro-Casp3 32 kD
Interaction
Active heterodimers between large p17 subunit and small p11 subunit of Casp7 and vice versa. Cleaves PARP, huntingtin, androgen receptor, atrophin-1, DNA- PKcs, D4-GDI, hnRNPs C1/C2, U1snRNP, PKC delta, ATM, fodrin, gelsolin, ICAD, RIP, X-IAP, STAT1, topo
Bioactivity
Involved in caspase activation cascade responsible for apoptosis execution. Cleaves/activates SREBPs, Casp6, Casp7, Casp9
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
Antigen References

1. Goldberg Y, et al. 1996. Nat. Genet. 13:442.
2. Fernandes-Alnemri T, et al. 1996. P. Natl. Acad. Sci. USA 93:7464.
3. Slee E, et al. 2001. J. Biol. Chem. 276:7320.
4. Woo M, et al. 2003. Nat. Immunol. 4:1016.

Regulation
Inhibited by dominant negative isoform, c-IAP1, c-IAP2, XIAP, survivin, Livin. Activated when cleaved by Casp6, -8, -10, granzyme B, Apaf-1 into large p17 and small p12 subunits
Gene ID
12367 View all products for this Gene ID
UniProt
View information about Caspase-3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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