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Purified anti-Catalase Antibody

Pricing & Availability
Clone
BL28633 (See other available formats)
Regulatory Status
RUO
Other Names
CAT, Epididymis Secretory Sperm Binding Protein
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
BL28633_Purified_Catalase_Antibody_1_053119
Western blot of purified anti-Catalase antibody (BL28633). Lane 1: Molecular weight marker; Lane 2: 20 µg of human liver lysate; Lane 3: 20 µg of mouse liver lysate; Lane 4: 20 µg of rat liver lysate. The blot was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • BL28633_Purified_Catalase_Antibody_1_053119
    Western blot of purified anti-Catalase antibody (BL28633). Lane 1: Molecular weight marker; Lane 2: 20 µg of human liver lysate; Lane 3: 20 µg of mouse liver lysate; Lane 4: 20 µg of rat liver lysate. The blot was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • BL28633_Purified_Catalase_Antibody_2_053119
    IHC staining of purified anti-Catalase antibody (BL28633) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • BL28633_Purified_Catalase_Antibody_3_053119
    IHC staining of purified anti-Catalase antibody (BL28633) on formalin-fixed paraffin-embedded mouse liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • BL28633_Purified_Catalase_Antibody_4_053119
    IHC staining of purified anti-Catalase antibody (BL28633) on formalin-fixed paraffin-embedded rat liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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863301 25 µg £73
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Description

Catalase is a heme-containing homotetrameric protein that decomposes hydrogen peroxide to water and oxygen to prevent the generation of hydroxyl radicals in cells. The role of catalase in cellular oxidative stress defense has been widely studied. Overexpression of catalase renders cells more resistant to hydrogen peroxide-induced toxicity and oxidant-mediated injury from hypoxia. Transgenic mice overexpressing catalase are protected against myocardial injury following adriamycin treatment. Although catalase knockout mice develop normally, they show differential sensitivity to oxidant tissue injury. Brain is particularly vulnerable to oxidative stress. Abnormalities and dysfunction of catalase have been indicated in neurodegenerative disorders such as Parkinson’s and Alzheimer’s diseases. Catalase activity was shown to be reduced in Parkinson-derived substantia nigra and putamen tissues. In in vitro cellular models of Alzheimer’s disease, aggregated amyloid beta demonstrates high affinity for catalase leading to the accumulation of hydrogen peroxide and increased oxidative stress.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Catalase
Formulation
0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 2.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2810758 (BioLegend Cat. No. 863301)

Antigen Details

Structure
Human Catalase is a 527 amino acid protein with a molecular mass of 60 kD.
Distribution

Tissue distribution: Ubiquitously expressed; highly expressed in bone marrow and liver.
Cellular distribution: Peroxisome and nucleus

Function
Catalase is an antioxidant enzyme involved in the body defense against oxidative stress.
Cell Targets
Hydrogen peroxide.
Cell Type
Astrocytes, Neurons
Biology Area
Cell Biology, Cell Death, Neurodegeneration, Neuroscience
Molecular Family
Enzymes and Regulators
Antigen References
  1. Ho YS, et al. 2004. J Biol Chem. 279(31):32804-12. 
  2. Ambani LM, et al. 1975. Arch Neurol. 32(2):114-8.
  3. Milton NG. 1999. Biochem J. 344(Pt 2): 293–296.
  4. Habib LK, et al. 2010. J Biol Chem.  285(50):38933-43
Gene ID
547 View all products for this Gene ID
UniProt
View information about Catalase on UniProt.org
Go To Top Version: 1    Revision Date: 05/31/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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