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Purified anti-Cathepsin A Antibody

Pricing & Availability
Clone
15D2C93 (See other available formats)
Regulatory Status
RUO
Other Names
CTSA, GSL, Protective Protein For Beta-Galactosidase (PPGB), GLB2, NGBE, Protective Protein Cathepsin A (PPCA), Carboxypeptidase C, Lysosomal Protective Protein, Lysosomal Carboxypeptidase A, Carboxypeptidase-L
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
15D2C93_PURE_CathepsinA_Antibody_WB_022516
20 or 50 ng of human Cathepsin A recombinant protein was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-Cathepsin A (clone 15D2C93) antibody. Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection.
  • 15D2C93_PURE_CathepsinA_Antibody_WB_022516
    20 or 50 ng of human Cathepsin A recombinant protein was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-Cathepsin A (clone 15D2C93) antibody. Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection.
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683902 100 µg £182
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Description

Cathepsin A, also known as Protective Protein Cathepsin A (PPCA), is a serine carboxypeptidase that is required for correct lysosomal transportation. Cathepsin A is synthesized as a 54 kD zymogen and is processed into an enzymatically active two-chain form in lysosomes. Cathepsin A exhibits carboxypeptidase activity at acidic pH in the lysosome and acts such as deaminase and esterase at a neutral pH. It participates in the inactivation of several selected neuropeptides, which includes substance P, oxytocin, and endothelin I. Cathepsin A forms a multienzyme complex with β-galactosidase (GLB1) and neuraminidase (NEU1). It is necessary for the stability and activity for these two enzymes. Genetic defects in the CTSA gene lead to the secondary combined deficiency of GLB1 and NEU1 and cause the lysosomal storage disease galactosialidosis (GS). Cathepsin A has also been suggested to regulate chaperone-mediated autophagy through the proteolytic inactivation of Lysosome-associated membrane protein 2 (LAMP2).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial Cathepsin A recombinant protein (29 - 480 a.a.) expressed in 293E cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2572060 (BioLegend Cat. No. 683902)

Antigen Details

Structure
480 amino acids with a predicted molecular weight of 54.5 kD. Processed active form of Cathepsin A contains a 32 kD chain and a 20 kD chain. These chains form a disulfide-linked heterodimer.
Distribution
Lysosome.
Function
Cathepsin A is a serine carboxypeptidase required for the activation and stabilization of β-galactosidase and neuraminidase in the lysosomal compartment. Defects in the gene can cause the lysosomal storage disorder galactosialidosis.
Interaction
Cathepsin A interacts with CTNNBIP1, GABARAP, ELN, and VAPA.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance
Molecular Family
Enzymes and Regulators, Lysosomal Markers
Antigen References

1. Kolli N, et al. 2014. J. Biol. Chem. 189:11592.
2. Takiguchi K, et al. 2000. J. Hum. Genet. 45:200.
3. Hahn CN, et al. 1998. Proc. Natl. Acad. Sci. USA 95:14880.
4. van der Spoel A, et al. 1998. EMBO J. 17:1588.
5. Rudenko G, et al. 1998. Proc. Natl. Acad. Sci. USA 95:621.
6. Galjart NJ, et al. 1991. J. Biol. Chem. 266:14754.

Gene ID
5476 View all products for this Gene ID
UniProt
View information about Cathepsin A on UniProt.org
Go To Top Version: 1    Revision Date: 02/25/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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