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Purified anti-Cathepsin D Antibody

Pricing & Availability
Clone
16E12C58 (See other available formats)
Regulatory Status
RUO
Other Names
CTSD, CPSD, CLN10, HEL-S-130P
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
16E12C58_PURE_CathepsinD_Antibody_WB_063015
Total cell lysate from human MCF-7 (lane 1) and Raw264.7 cells (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-Cathepsin D (clone 16E12C58) antibody. Proteins were visualized using an HRP-conjugated anti-mouse-IgG secondary and chemiluminescence detection.
  • 16E12C58_PURE_CathepsinD_Antibody_WB_063015
    Total cell lysate from human MCF-7 (lane 1) and Raw264.7 cells (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-Cathepsin D (clone 16E12C58) antibody. Proteins were visualized using an HRP-conjugated anti-mouse-IgG secondary and chemiluminescence detection.
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678502 100 µg £182
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Description

Cathepsin D (CTSD) is a major lysosomal aspartic endoproteinase. It is active in acidic conditions with a pH range from 3.5 to 5.0, and its activity is inhibited by pepstatin A. CTSD is involved in lysosomal protein turnover and many other cellular processes. CTSD participates in the resolution of inflammation by initiating the apoptotic program in neutrophils. CTSD is released from neutrophil azurophilic granules during the initial stage of apoptosis, which leads to death receptor-independent activation of caspase-8. CTSD induces apoptosis in other cells as well. In T cells, CTSD activates Bax, which leads to the release of mitochondrial apoptosis-inducing factor (AIF), and subsequently induces an early caspase-independent apoptotic phenotype. CTSD also takes part in the release of cytochrome C from the mitochondria and activation of caspases in fibroblast apoptosis, which is induced by staurosporine. In addition, CTSD participates in cell proliferation, and it also increases proliferation and metastatic potential of many cancer cell lines. Overexpression of CTSD has been reported in many different cancers including malignant glioma, melanoma, breast, ovarian, endometrial, and bladder cancer. CTSD is also involved in neurodegenerative diseases. CTSD knockout mice die shortly after birth and display some neurodegeneration phenotypes. Mutations of CTSD cause congenital neuronal ceroid lipofuscinosis in humans and animals. CTSD cleaves the amyloid precursor protein and may play a role in Alzheimer’s disease. CTSD-deficiency has also been associated with Parkinson disease. Like CTSB and CTSL, CTSD functions outside the cells and is able to cleave extracellular matrix proteins including collagen, fibronectin, proteoglycans, and laminin.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human cathepsin D recombinant protein (21-412 a.a.) expressed in 293E cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2565905 (BioLegend Cat. No. 678502)

Antigen Details

Structure
The 402 amino acid recombinant protein has a predicted molecular mass of approximately 44 kD. Aspartic endoproteinase.
Distribution
Lysosome. Secreted. CTSD is found in most cells and tissues of mammals. It is very abundant in macrophages.
Function
Lysosomal protein turnover, apoptosis, cell proliferation, cell invasion, neurodegeneration, and immune responses. Upregulated by estradiol, insulin, insulin-like growth factor, EGF, and FGF basic.
Ligand/Receptor
Caspase-8, collagen, fibronectin, proteoglycans, and laminin.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance
Molecular Family
Enzymes and Regulators, Lysosomal Markers
Antigen References

1. Vetvicka V, et al. 1994. Cancer Lett. 79:131.
2. Johansson AC, et al. 2003. Cell Death Differ. 10:1253.
3. Cavailles V, et al. 1988. Nucleic Acids Res. 16:1903.
4. Bidere N, et al. 2003. J. Biol. Chem. 278:31401.
5. Conus S, et al. 2012. J. Biol. Chem. 25:21142.
6. Koike M, et al. 2001. J. Neurosci. 21:7526.
7. Cullen V, et al. 2009. Mol. Brain 2:5.
8. Dian D, et al. 2014. Histol. Histopathol. 29:433.

Gene ID
1509 View all products for this Gene ID
UniProt
View information about Cathepsin D on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06/30/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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