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Purified anti-Cav1.2 Antibody

Pricing & Availability
Clone
N263/31 (See other available formats)
Regulatory Status
RUO
Other Names
Calcium Voltage-Gated Channel Subunit Alpha1 C, Cav1.2, CACH2, CACN2, CACNL1A1, LQT8, TS, DHPR Alpha-1 Subunit , DHPR, Alpha-1 Subunit
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
N263slash31_PURE_Cav1dot2_Antibody_102021
IHC staining of purified anti-Cav1.2 antibody (clone N263/31) on mouse brain. Tissue was fixed with Fixation Buffer (Cat. No. 420801), blocked with 5% FBS + 5% Normal Blocking Serum (Cat. No. 927503), and incubated with 10.0 µg/mL purified mouse IgG2b, κ isotype ctrl (Cat. No. 400302) (panel A) or 5.0 µg/mL of the primary antibody (panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) at 2.5 µg/mL. Nuclei were counterstained with DAPI (blue) and the slides were mounted with ProLong™ Gold Antifade Mountant. The images were captured with a 40X objective. Scale: 50 µm.
  • N263slash31_PURE_Cav1dot2_Antibody_102021
    IHC staining of purified anti-Cav1.2 antibody (clone N263/31) on mouse brain. Tissue was fixed with Fixation Buffer (Cat. No. 420801), blocked with 5% FBS + 5% Normal Blocking Serum (Cat. No. 927503), and incubated with 10.0 µg/mL purified mouse IgG2b, κ isotype ctrl (Cat. No. 400302) (panel A) or 5.0 µg/mL of the primary antibody (panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) at 2.5 µg/mL. Nuclei were counterstained with DAPI (blue) and the slides were mounted with ProLong™ Gold Antifade Mountant. The images were captured with a 40X objective. Scale: 50 µm.
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601952 100 µg £113
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Description

Cav1.2 is a subunit of the L-type voltage-dependent calcium channel, which regulates calcium influx in response to membrane depolarization. Cav1.2 forms the calcium-selective pore, in addition to the critical voltage sensor and most drug interaction sites. Through these functions, Cav1.2 mediates a number of calcium-dependent cellular processes including neuron excitation, muscle contraction, and neuroendocrine cell secretion. Mutations in Cav1.2 underlie several diseases, notably Timothy’s syndrome and Brugada syndrome, which are cardiac arrhythmias. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Fusion protein corresponding to amino acids 808-874 of recombinant rat Cav1.2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-F - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2904446 (BioLegend Cat. No. 601952)

Antigen Details

Structure
Cav1.2 is a 2,221 amino-acid protein with a predicted molecular weight of 249 kD.
Distribution

Expressed in neurons, cardiomyocytes, and smooth muscle cells

Function
Voltage-gated calcium channel
Molecular Family
Ion Channels
Antigen References
  1. Hofmann F, et al. 2014. Physiol. Rev. 91:1 303-326. 
Gene ID
775 View all products for this Gene ID
UniProt
View information about Cav1.2 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 10/20/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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