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Purified anti-Cavβ4 Antibody

Pricing & Availability
Clone
N10/7 (See other available formats)
Regulatory Status
RUO
Other Names
Cavβ4, Calcium Voltage-Gated Channel Auxiliary Subunit Beta 4, Voltage-Dependent L-Type Calcium Channel Subunit Beta-4, Calcium Channel Voltage-Dependent Subunit Beta 4
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
N107_PURE_Cav4_Antibody_110921
Whole tissue membrane extracts (15 µg total protein) from human, rat, and mouse brain tissue were heated at 37°C for 30 minutes, resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL of purified anti-Cavβ4 antibody (clone N10/7) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker
  • N107_PURE_Cav4_Antibody_110921
    Whole tissue membrane extracts (15 µg total protein) from human, rat, and mouse brain tissue were heated at 37°C for 30 minutes, resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL of purified anti-Cavβ4 antibody (clone N10/7) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker
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602452 100 µg £113
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Description

Voltage-dependent L-type calcium channel subunit beta-4 (Cavß4) is a member of the membrane-associated guanylate kinase (GK) protein family and a cytoplasmic subunit of voltage-gated calcium channels. Expressed predominantly in the cerebellum and kidney, it is responsible for calcium entry into nerve termini and subsequent neurotransmitter release via its association with membrane-trafficking protein synaptotagmin 1 (Syt 1). It regulates synaptic transmission, increases the readily releasable pool of synaptic vesicles, and plays a role in synaptic vesicle plasticity and maturation through its association with Cavß1, RIM1, and actin filaments (F-actin). Cavß4 has been linked to psychiatric and anxiety disorders including major depressive disorder (MDD) and schizophrenia and is a potential therapeutic target in their study and treatment.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide corresponding to amino acids 458-474 of rat Cavβ4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone has been tested for IHC and failed.

RRID
AB_2904448 (BioLegend Cat. No. 602452)

Antigen Details

Structure
Cavβ4 is a 520 amino acid protein with a predicted molecular weight of 58 kD.
Distribution

Ubiquitously expressed but predominantly expressed in brain, cytosol and plasma membrane.

Function
Calcium channel, voltage-gated channel, ion channel
Molecular Family
Ion Channels, Neurotransmitter Receptors
Antigen References
  1. Andrade A, et al. 2019. Int. J. Mol. Sci. 20:3537.
  2. Gustavo G, et al. 2019. Front. Cell. Neurosci. 13:125.
  3. Weiss N. 1999. J. Neurosci. 23:6117.
Gene ID
785 View all products for this Gene ID
UniProt
View information about Cavbeta4 on UniProt.org
Go To Top Version: 1    Revision Date: 11/09/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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