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Purified anti-CD106 (VCAM-1) Antibody (Previously Covance catalog# MMS-476P)

Pricing & Availability
Clone
P3C4 (See other available formats)
Regulatory Status
RUO
Other Names
Vascular cell adhesion protein 1, CD106 antigen
Previously
Covance Catalog# MMS-476P
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P3C4_VCAM-1_CD106_Purified_IHC_031615
Staining of VCAM-1 (Clone P3C4) on frozen ovarian tumor tissue.
  • P3C4_VCAM-1_CD106_Purified_IHC_031615
    Staining of VCAM-1 (Clone P3C4) on frozen ovarian tumor tissue.
  • P3C4_VCAM-2_CD106_Purified_IHC_031521
    IHC staining of purified anti-CD106 (VCAM-1) antibody (clone P3C4) on frozen human ovarian tumor tissue. The tissue was incubated with 1.0 µg/mL of the primary antibody 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929501) was used for detection followed by hemotoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
Cat # Size Price Quantity Check Availability Save
919801 100 µL £189
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Description

CD106 is a 110 kD single chain type I glycoprotein, which is also known as VCAM-1 and INCAM-110. It is expressed predominantly on activated vascular endothelium but has also been identified on follicular and interfollicular dendritic cells, some macrophages, bone marrow stromal cells, and non-vascular cell populations within joints, kidney, muscle, heart, placenta, and brain. Expression on endothelial cells as well as many other cells is induced by inflammatory stimuli and cytokines. Activated endothelial cells can release soluble forms of CD106 which can be detected in the blood. CD106 binds the integrins CD49d/CD29 (VLA-4) and α4β7 that contribute to leukocyte adhesion, transmigration, and co-stimulation of T cell proliferation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
The P3C4 monoclonal antibody was generated against IL-1β activated human umbilical vein endothelial cells (HUVECs).
Formulation
Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-F - Quality tested
IP, ICC, FC
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections.

The optimal working dilution should be determined for each specific assay condition.
• IF: 1:1,000
• IP: 1:1,000
• FC: 1:1,000
• IHC: 1:500 - 1:1000 using an AcuityAdvanced Kit available in the BioLegend Antibody Products web store

Tissue: Frozen sections
Pretreatment: None 
Primary Incubation: 60 minutes at RT

Application Notes

This antibody is effective in immunohistochemistry (IHC), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FC), but only on IL-1ß activated cells.

IHC Positive Control: Human Ovarian Tumor

This antibody inhibits VCAM-1 binding by a4ß1. When used with another monoclonal antibody directed to an alternative epitope this antibody can be used to capture soluble serum VCAM. 

Application References

(PubMed link indicates BioLegend citation)
  1. Kim JA, et al. 1994. Arterioscler Thromb. 14:427.
RRID
AB_2565212 (BioLegend Cat. No. 919801)

Antigen Details

Cell Type
Mesenchymal Stem Cells
Biology Area
Cell Biology, Neuroinflammation, Neuroscience, Signal Transduction, Stem Cells, Transcription Factors
Molecular Family
Adhesion Molecules, CD Molecules
Gene ID
7412 View all products for this Gene ID
UniProt
View information about CD106 on UniProt.org
Go To Top Version: 5    Revision Date: 06/10/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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