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Purified anti-CD117 (c-KIT) Phospho (Tyr703) Antibody

Pricing & Availability
Clone
A22001B (See other available formats)
Regulatory Status
RUO
Other Names
KIT, Mast/Stem Cell growth factor receptor Kit, CD117, C-KIT, PBT, Tyrosine-Protein Kinase Kit, SCFR, V-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A22001B_PURE_CD117_Antibody_110923
Whole cell extracts (15 µg total protein) from HEL cells untreated or treated with HSCF (50 ng/mL, 5 minutes), were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL of purified anti-CD117 Phospho (Tyr703) (clone A22001B) (left panel) or anti-CD117 Pan (right panel) for 2 hours. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • A22001B_PURE_CD117_Antibody_110923
    Whole cell extracts (15 µg total protein) from HEL cells untreated or treated with HSCF (50 ng/mL, 5 minutes), were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL of purified anti-CD117 Phospho (Tyr703) (clone A22001B) (left panel) or anti-CD117 Pan (right panel) for 2 hours. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
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619551 25 µg £121
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619552 100 µg £301
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Description

Proto-oncogene CD117/c-KIT is a type III receptor tyrosine kinase that binds Stem Cell Factor (SCF). CD117 is used to identify certain types of hematopoietic progenitors in bone marrow, such as hematopoietic stem cells (HSC), multipotent progenitors (MPP), and common myeloid progenitors (CMP). Expression of CD117/c-KIT is also found in stem cell precursors from prostate, liver, and heart. CD117/c-KIT signaling plays a role in cell survival, proliferation, and differentiation; hence, its activation is required for melanocyte survival, hematopoiesis and gametogenesis. Aberrant activation of CD117/c-KIT due to gene mutations has been associated with gastrointestinal stromal tumors, testicular seminoma, mast cell disease, melanoma, acute myeloid leukemia, and others, while loss of activation is associated with piebaldism.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide from human CD117 (c-KIT) phosphorylated at Tyr703.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3083404 (BioLegend Cat. No. 619551)
AB_3083404 (BioLegend Cat. No. 619552)

Antigen Details

Structure
c-KIT is a 976 amino acid protein with a predicted molecular weight of 110 kD. It is a type III receptor tyrosine kinase protein that is composed of five immunoglobulin-like domains on the extracellular side and an intracellular tyrosine kinase domain.
Distribution

c-KIT is a transmembrane protein but can also be found in cytoplasmic vesicles after internalization.

Function
Regulation of cell survival and proliferation, Hematopoiesis, Stem cell maintenance, Gametogenesis, Mast cell development, Melanogenesis
Interaction
Activate AKT1 via phosphorylation of PIK3R1, activate RAS, RAF1, and MAP kinases, promote activation of STAT family members
Ligand/Receptor
c-KIT is the receptor for Mast/Stem cell growth factor (SCF)
Biology Area
Cancer Biomarkers, Cell Biology, Immuno-Oncology
Molecular Family
CD Molecules, Phospho-Proteins
Antigen References
  1. Myburgh R, et al. 2020. Leukemia. 34:2688-703.
  2. Miettinen M, et al. 2005. Appl Immunohistochem Mol Morphol. 13:205-20.
  3. Cananzi M, et al. 2012. Organogenesis. 8:77-88.
  4. Harris KS, et al. 2021. Sci Rep. 11:1465.
Gene ID
3815 View all products for this Gene ID
UniProt
View information about CD117 on UniProt.org
Go To Top Version: 1    Revision Date: 06/21/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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