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Purified anti-CD141 (Thrombomodulin) Antibody (Previously Covance catalog# MMS-176P)

Pricing & Availability
Clone
Phx-01 (See other available formats)
Regulatory Status
RUO
Other Names
Thrombomodulin, Fetomodulin, CD141 antigen, AHUS6, BDCA3, THPH12, THRM
Previously
Covance Catalog# MMS-176P
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
Phx-01_Purified_CD141_Antibody_IHC_021315
IHC staining of Phx-01, MMS-176P on frozen human placenta.
  • Phx-01_Purified_CD141_Antibody_IHC_021315
    IHC staining of Phx-01, MMS-176P on frozen human placenta.
  • Phx-01_Purified_CD141_Antibody_WB_112817
    Total lysates (15 µg protein) from HL-60 (low expression negative control), HeLa and MCF-7 (positive control) were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1 µg/ml) purified anti-CD141 antibody, clone Phx-01. Proteins were visualized using chemiluminescence detection by incubation with HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-GAPDH was used as a loading control (Cat. No. 649203, 1:1000 dilution).
Cat # Size Price Quantity Check Availability Save
902102 25 µL £61
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902101 100 µL £174
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Description

CD141 is a 75 kD, single chain, type I membrane glycoprotein also known as thrombomodulin, TM, THRM, THBD, and fetomodulin. CD141 is an important cofactor in the protein C anticoagulant system. After binding to its ligand thrombin, CD141 activates protein C, which degrades clotting factors Va and VIIIa, and as a consequence the amount of thrombin is reduced. CD141 is expressed on macrophages, monocytes, a subpopulation of myeloid dendritic cells, vascular endothelial cells, and keratinocytes. Besides anti-coagulation function, CD141 is also involved in embryonic and atherosclerotic plaque development.  CD141 is an endothelial cell surface glycoprotein involved in the regulation of coagulation. Via a direct interaction with thrombin, thrombomodulin causes down-regulation of multiple pro-coagulation pathways in order to preserve the anticoagulant properties of the endothelial cell surface.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Other species
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution & 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-F - Quality tested
WB, ELISA - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
• WB: 1 - 2 µg/ml (1:500 - 1:1000)
• ELISA: 1:1,000
• IHC: 1:100

Tissue Sections: Frozen tissue sections
Incubation: 60 minutes at room temperature

Application Notes

This antibody is effective in immunoblotting, immunohistochemistry (IHC) and ELISA.

*Predicted MW = 60 kD

Positive control tissue: human placenta

This antibody reacts with an epitope within the EGF 5 domain and inhibits thrombin binding.

Application References

(PubMed link indicates BioLegend citation)
  1. Nagashima M, et al. 1993. J Biol Chem. 268:2888.
  2. Parkinson JF, et al. 1992. Biochem Biophys Res Commun. 185:567
Product Citations
  1. Gruber CN, et al. 2020. Cell. 183:982. PubMed
RRID
AB_2565016 (BioLegend Cat. No. 902102)
AB_2565016 (BioLegend Cat. No. 902101)

Antigen Details

Structure
Single chain, type I membrane glycoprotein, 75 kD
Distribution

Macrophages, monocytes, subset of myeloid dendritic cells, vascular endothelial cells, keratinocytes

Function
After thrombin binding, CD141 activates protein C, which degrades clotting factors Va and VIIIa and reduces the amount of thrombin generated.
Interaction
Protein C, Thrombin-activatable fibrinolysis inhibitor (TAFI), Platelet factor 4 (PF4)
Ligand/Receptor
Thrombin
Cell Type
Dendritic cells, Endothelial cells, Macrophages, Monocytes
Biology Area
Cell Biology, Neuroinflammation, Neuroscience, Signal Transduction
Gene ID
7056 View all products for this Gene ID
UniProt
View information about CD141 on UniProt.org
Go To Top Version: 4    Revision Date: 09/22/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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