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Purified anti-CD209/CD299 (DC-SIGN/L-SIGN) Antibody

Pricing & Availability
Clone
14E3G7 (See other available formats)
Regulatory Status
RUO
Other Names
For CD209: CDSIGN, CLEC4L, DC-SIGN, DC-SIGN1, For CD299: LSIGN, CD209L, L-SIGN, DCSIGNR, HP10347, DC-SIGN2, DC-SIGNR
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
14E3G7_PURE_CD209slashCD299_Antibody_FC_072915
Human monocyte-derived dendritic cells were stained with purified CD209/CD299 (DC-SIGN/L-SIGN) (clone 14E3G7) (filled histogram) or purified mouse IgG2b, κ isotype control (open histogram), and then was followed by anti-mouse IgG PE.
  • 14E3G7_PURE_CD209slashCD299_Antibody_FC_072915
    Human monocyte-derived dendritic cells were stained with purified CD209/CD299 (DC-SIGN/L-SIGN) (clone 14E3G7) (filled histogram) or purified mouse IgG2b, κ isotype control (open histogram), and then was followed by anti-mouse IgG PE.
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845002 100 µg £182
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Description

DC-SIGN is a C-type lectin that has a high affinity for the ICAM3 molecule. It binds various microorganisms by recognizing high-mannose-containing glycoproteins on their envelopes. It especially functions as a receptor for several viruses such as HIV and Hepatitis C. L-SIGN is a transmembrane receptor and is referred to as L-SIGN because of its expression in the endothelial cells of the lymph nodes and liver. The protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain, and a C-type lectin carbohydrate recognition domain.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
DC-SIGN DNA.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 14E3G7 has been shown to recognize both CD209 and CD299 transfectants.1

Application References

(PubMed link indicates BioLegend citation)
  1. Granelli-Piperno A, et al. 2005. J. Immunol. 175:4265.
Product Citations
  1. Lempp FA, et al. 2021. Nature. 598:342. PubMed
RRID
AB_2566004 (BioLegend Cat. No. 845002)

Antigen Details

Structure
DC-SIGN is a 44 kD type II transmembrane glycoprotein and a member of the C-type lectin family. DC-SIGNR (DC-SIGN-related molecule) is a type II integral membrane protein that is 77% identical to DC-SIGN.
Distribution

CD209 is expressed on myeloid dendritic cells, placental macrophages, and liver and placental endothelial cells. CD299 is expressed on liver sinusoidal endothelial cells, lung and placental endothelial cells, type 2 alveolar cells, and endothelial cells of lymph nodes.

Function
CD299 mediates interactions between dendritic cells and resting T cells and has a high affinity for HIV-1 gp120. CD209 plays a critical role in capturing and internalizing pathogens. LSP1 (leukocyte-specific protein 1) interacts with the cytoplasmic domain of CD209 and mediates the transport of HIV to the proteasome.
Interaction
CD209 is a receptor of viruses such as HIV-1, West Nile Virus, hepatitis C virus, some bacteria, and parasites. CD299 binds carbohydrate structures of pathogens such as hepatitis C virus, Mycobacterium, etc.
Ligand/Receptor
CD209 binds to ICAM-3 (CD50), ICAM-2 (CD102), and Butyrophilin (BTNA2A1). CD299 is a receptor for ICAM-3 (CD50).
Cell Type
Dendritic cells, Endothelial cells, Macrophages
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Fauci A. 1996. Nature 384:529.
2. Yokoyama-Kobayashi M, et al. 1999. Gene 228:161.
3. Soilleux EJ, et al. 2000. J. Immunol. 165:2937.
4. Pohlmann S, et al. 2001. Proc. Natl. Acad. Sci. USA 98:2670.
5. Bashirova AA, et al. 2001. J. Exp. Med. 193:671.
6. Lai WK, et al. 2006. Am. J. Pathol. 169:200.

Gene ID
30835 View all products for this Gene ID 10332 View all products for this Gene ID
UniProt
View information about CD209 on UniProt.org
Go To Top Version: 1    Revision Date: 07/29/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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