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Purified anti-CD243/ABCB4 (ABCB1, P-glycoprotein1/MDR3) Antibody (Previously Covance catalog# SIG-38710)

Pricing & Availability
Clone
C219 (See other available formats)
Regulatory Status
RUO
Other Names
Multidrug resistance protein 3, P-glycoprotein 3, multiple drug resistance 3, ATP-binding cassette sub-family B member 4, P glycoprotein 3/multiple drug resistance 3
Previously
Signet Catalog# 8710-01
Covance Catalog# SIG-38710
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
a-C219_Purified_C219_Antibody_IHC_032519_updated
IHC staining of purified anti-CD243/ABCB4 (ABCB1, P-glycoprotein1/MDR3antibody (clone C219) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 1: Universal, 100X (Cat. No. 927901), the tissue was incubated with a 1:20 dilution of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • a-C219_Purified_C219_Antibody_IHC_032519_updated
    IHC staining of purified anti-CD243/ABCB4 (ABCB1, P-glycoprotein1/MDR3antibody (clone C219) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 1: Universal, 100X (Cat. No. 927901), the tissue was incubated with a 1:20 dilution of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • b-C219_PURE_CD243-ABCB4_Antibody_2_031820
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a 1:100 dilution of purified anti-CD243/ABCB4 antibody (clone C219) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • c-C219_PURE_CD243-ABCB4_Antibody_3_031820
    Whole cell extracts (15 µg total protein) from the indicated cell lines and tissue were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a 1:100 dilution of purified anti-CD243/ABCB4 antibody (clone C219) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
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903701 1 mL £414
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Description

Clone C219 recognizes an internal, highly conserved amino acid sequence (VQEALD & VQAALD) found in both MDR1 and MDR3 isoforms of P-glycoprotein. The specific binding of the clone C219 was stronger in epitope sequence VQAALD (N-Terminal fragment) than the epitope sequence VQEALD (C-terminal fragmant) (Georges E., et al., 1990). Analysis of p-glycoprotein expression in skeletal and cardiac muscle revealed that C219 cross reacts with a 200kDa protein that migrates in the same position as myosin. C219 has also been reported to detect the 185kDa c-erbB2 protein (Liu, et al., 1997). Care should be taken in the interpretation of true positive P-glycoprotein expression when using myosin-containing tissues or tissues known to have p185 c-erbB2 expression.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Reported Reactivity
Rat, Dog, Non-Human Primate
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was made against SDS-solubilized plasma membranes of a multidrug resistant Chinese hamster ovary (CHO) cell line and a human cell line.
Formulation
Phosphate-buffered solution with BSA + 0.09% NaN3.

Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation
The antibody was purified by affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Store between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
IHC-F, ICC, FC, WB, ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution range of 1:20 - 1:40 is suggested.  For western blotting, the suggested use of this reagent is a 1:100 dilution. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Hisano Y, et al. 2011. J. Biol. Chem. 286: 1758. (WB)
  2. Beck WT, et al. 1996. Cancer Res. 56:3010. (IHC-P, IHC-F, ICC, FC) PubMed
  3. Evans CH, Baker PD. 1992. Cancer Res. 52: 5893. (WB, FC)
  4. Toth K, et al. 1992. Am J Pathol. 140:1009. (IHC-P, ICC, WB)
  5. Finstad CL, et al. 1991. J Histochem Cytochem. 39:1603. (FC, WB, IHC-P
  6. Georges E, et al. 1990. PNAS. 87:152. (ELISA, IHC-P)
  7. Duan Z, et al. 2014. BMC Cancer. 14:681. (WB)
  8. Inui N, et al. 2012. J Gerontol A Biol Sci Med Sci. 67:1049. (FC, IF)
  9. Erickson MA, et al. 2012. J Neuroinflammation. 9:150. (WB)
  10. Rao VV, et al. 1994. Cancer Res. 54:1536. (WB) PubMed
  11. Lee JJ, et al. 1996. Cancer. 77:1892. (IHC) PubMed
Product Citations
  1. Giddings EL, et al. 2021. Nat Commun. 12:2804. PubMed
  2. Lee J, et al. 1996. Cancer. 77:1892-1898. PubMed
  3. Afrouzian M, et al. 2018. Biochem Pharmacol. 156:467. PubMed
  4. Sophie Kang S, et al. 2016. PLoS One. 11:e0165638. PubMed
  5. Beck W, et al. 1996. Cancer Res. 56:3010-3020. PubMed
  6. Song YK, et al. 2019. J Pharmacol Exp Ther. 368:79. PubMed
RRID
AB_2565033 (BioLegend Cat. No. 903701)

Antigen Details

Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Gene ID
5244 View all products for this Gene ID
UniProt
View information about CD243/ABCB4 on UniProt.org
Go To Top Version: 8    Revision Date: 02/08/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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